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10.1016/j.bbacli.2014.06.002 http://scihub22266oqcxt.onion/10.1016/j.bbacli.2014.06.002 C4633918!4633918!26673636     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BBA+Clin 2014 ; 1 (ä): 52-8 Nephropedia Template TP {{tp|p=26673636|t=2014. Enumeration of circulating fibrocytes for clinical use in asthma by an optimized single-platform flow cytometry assay |pdf=|usr=}}{{26673636}} gab.com Text Enumeration of circulating fibrocytes for clinical use in asthma by an optimized single-platform flow cytometry assay JO: BBA Clin http://www.kidney.de/pget.php?&tw=1&pmid=26673636 #FOAvip Background: Elevated numbers of circulating fibrocytes are associated with inadequately controlled asthma, poor response to available therapies, and increased risk of adverse outcomes. The lack of reliable and clinically-applicable assays precludes a proper evaluation of blood fibrocyte count as a prognostic biomarker in asthma. This report concerns the use of a multiparameter flow cytometry assay for the enumeration of fibrocytes in the whole blood. Methods: Consenting fibrocyte donors were 19 patients with asthma well controlled by current treatment, 16 patients with treatment-resistant asthma, 9 patients with transiently uncontrolled asthma and 14 age-matched normal individuals. Blood sampling was performed once in patients with transiently uncontrolled asthma and twice, at an interval of one week, in the other subjects. The assay was performed in 100 ?l of whole blood and involved a sequential gating strategy and absolute fibrocyte counting with a single instrument (single-platform assay). Results: The quantification of circulating fibrocytes by this assay was analytically and clinically valid. In individuals with stable clinical conditions, the repeatability of blood fibrocyte counts over one week was good. The intraclass correlation coefficient was 0.939 and 96.88% of the total variability reflected on-average differences among the tested subjects. Stabilized blood samples could be stored at 4 °C for up to 96 h before processing. Conclusions: The novel assay for the enumeration of fibrocytes in the whole blood is reliable and clinically applicable. General significance: This report demonstrates the validity and reliability of the first optimized assay for the enumeration of circulating fibrocytes in multicenter clinical trials. Twit Text FOAVip Enumeration of circulating fibrocytes for clinical use in asthma by an optimized single-platform flow cytometry assay ????BBA Clin http://www.kidney.de/pget.php?&tw=1&pmid=26673636 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26673636 #FOAvip English Wikipedia <ref>{{Cite pmid|26673636}}</ref> Enumeration of circulating fibrocytes for clinical use in asthma by an optimized single-platform flow cytometry assay #MMPMID26673636 Bianchetti L; Isgrò M; Marini MA; Bellini A; Schmidt M; Mattoli S BBA Clin 2014[Jun]; 1 (ä): 52-8 PMID26673636show ga Background: Elevated numbers of circulating fibrocytes are associated with inadequately controlled asthma, poor response to available therapies, and increased risk of adverse outcomes. The lack of reliable and clinically-applicable assays precludes a proper evaluation of blood fibrocyte count as a prognostic biomarker in asthma. This report concerns the use of a multiparameter flow cytometry assay for the enumeration of fibrocytes in the whole blood. Methods: Consenting fibrocyte donors were 19 patients with asthma well controlled by current treatment, 16 patients with treatment-resistant asthma, 9 patients with transiently uncontrolled asthma and 14 age-matched normal individuals. Blood sampling was performed once in patients with transiently uncontrolled asthma and twice, at an interval of one week, in the other subjects. The assay was performed in 100 ?l of whole blood and involved a sequential gating strategy and absolute fibrocyte counting with a single instrument (single-platform assay). Results: The quantification of circulating fibrocytes by this assay was analytically and clinically valid. In individuals with stable clinical conditions, the repeatability of blood fibrocyte counts over one week was good. The intraclass correlation coefficient was 0.939 and 96.88% of the total variability reflected on-average differences among the tested subjects. Stabilized blood samples could be stored at 4 °C for up to 96 h before processing. Conclusions: The novel assay for the enumeration of fibrocytes in the whole blood is reliable and clinically applicable. General significance: This report demonstrates the validity and reliability of the first optimized assay for the enumeration of circulating fibrocytes in multicenter clinical trials. äEnumeration of circulating fibrocytes for clinical use in asthma by an(epmc).pdf DeepDyve Pubget Overpricing