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Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model
Approach Based on Linear Fitting
#MMPMID26579547
Naegelen I
; Beaume N
; Plançon S
; Schenten V
; Tschirhart EJ
; Bréchard S
J Immunol Res
2015[]; 2015
(?): 817038
PMID26579547
show ga
Neutrophils participate in the maintenance of host integrity by releasing various
cytotoxic proteins during degranulation. Due to recent advances, a major role has
been attributed to neutrophil-derived cytokine secretion in the initiation,
exacerbation, and resolution of inflammatory responses. Because the release of
neutrophil-derived products orchestrates the action of other immune cells at the
infection site and, thus, can contribute to the development of chronic
inflammatory diseases, we aimed to investigate in more detail the spatiotemporal
regulation of neutrophil-mediated release mechanisms of proinflammatory
mediators. Purified human neutrophils were stimulated for different time points
with lipopolysaccharide. Cells and supernatants were analyzed by flow cytometry
techniques and used to establish secretion profiles of granules and cytokines. To
analyze the link between cytokine release and degranulation time series, we
propose an original strategy based on linear fitting, which may be used as a
guideline, to (i) define the relationship of granule proteins and cytokines
secreted to the inflammatory site and (ii) investigate the spatial regulation of
neutrophil cytokine release. The model approach presented here aims to predict
the correlation between neutrophil-derived cytokine secretion and degranulation
and may easily be extrapolated to investigate the relationship between other
types of time series of functional processes.
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