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2015 ; 16
(10
): 25560-75
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gab.com Text
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English Wikipedia
Novel Electrokinetic Microfluidic Detector for Evaluating Effectiveness of
Microalgae Disinfection in Ship Ballast Water
#MMPMID26516836
Maw MM
; Wang J
; Li F
; Jiang J
; Song Y
; Pan X
Int J Mol Sci
2015[Oct]; 16
(10
): 25560-75
PMID26516836
show ga
Ship ballast water treatment methods face many technical challenges. The
effectiveness of every treatment method usually is evaluated by using large scale
equipment and a large volume of samples, which involves time-consuming,
laborious, and complex operations. This paper reports the development of a novel,
simple and fast platform of methodology in evaluating the efficiency and the best
parameters for ballast water treatment systems, particularly in chemical
disinfection. In this study, a microfluidic chip with six sample wells and a
waste well was designed, where sample transportation was controlled by
electrokinetic flow. The performance of this microfluidic platform was evaluated
by detecting the disinfection of Dunaliella salina (D. salina) algae in ballast
water treated by sodium hypochlorite (NaClO) solution. Light-induced chlorophyll
fluorescence (LICF) intensity was used to determine the viability of microalgae
cells in the system, which can be operated automatically with the dimension of
the detector as small as 50 mm × 24 mm × 5 mm. The 40 µL volume of sample
solution was used for each treatment condition test and the validity of detection
can be accomplished within about five min. The results show that the viability of
microalgae cells under different treatment conditions can be determined
accurately and further optimal treatment conditions including concentrations of
NaClO and treatment time can also be obtained. These results can provide accurate
evaluation and optimal parameters for ballast water treatment methods.