Luo X
; Han M
; Liu J
; Wang Y
; Luo X
; Zheng J
; Wang S
; Liu Z
; Liu D
; Yang PC
; Li H
Sci Rep
2015[Nov]; 5
(?): 15937
PMID26526003
show ga
The aberrant immunity plays an important role in the pathogenesis of allergic
diseases. Micro RNAs (miR) are involved in regulating the immunity in the body.
This study aims to test a hypothesis that miR-146a induces the expression of
interleukin (IL)-10 in monocytes (Mos). In this study, the levels of miR-146a
were determined by real time RT-PCR. The IL-10(+) Mos were evaluated by flow
cytometry. The miR-146a-laden exosomes were generated with RPMI2650 cells (an
airway epithelial cell line). An allergic rhinitis mouse model was developed. The
results showed that nasal epithelial cells expressed miR-146a, which was markedly
lower in the nasal epithelial cells of patients with nasal allergy than that in
healthy controls. Exposure to T helper (Th)2 cytokines suppressed the levels of
miR-146a in the nasal epithelial cells. The nasal epithelial cell-derived
miR-146a up regulated the expression of IL-10 in Mos. The inducible IL-10(+) Mos
showed an immune suppressor effect on the activities of CD4(+) effector T cells
and the Th2 polarization in a mouse model of allergic rhinitis. In summary, nasal
epithelial cells express miR-146a, the latter is capable of inducing IL-10
expression in Mos, which suppress allergic reactions in the mouse nasal mucosa.
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