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10.1002/biot.201400422

http://scihub22266oqcxt.onion/10.1002/biot.201400422
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C4629492!4629492!25868062
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suck abstract from ncbi


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pmid25868062      Biotechnol+J 2015 ; 10 (9): 1360-9
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  • Dynamic metabolic engineering: New strategies for developing responsive cell factories #MMPMID25868062
  • Brockman IM; Prather KL
  • Biotechnol J 2015[Sep]; 10 (9): 1360-9 PMID25868062show ga
  • Metabolic engineering strategies have enabled improvements in yield and titer for a variety of valuable small molecules produced naturally in microorganisms, as well as those produced via heterologous pathways. Typically, the approaches have been focused on up- and downregulation of genes to redistribute steady-state pathway fluxes, but more recently a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium. This review highlights some of the recently published work related to dynamic metabolic engineering strategies and explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems. Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates. The implementation is more complex relative to static control, but advances in screening techniques and DNA synthesis will continue to drive innovation in this field.
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