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10.1007/s00018-014-1738-5 http://scihub22266oqcxt.onion/10.1007/s00018-014-1738-5 C4629475!4629475 !25274061     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25274061 &cmd=llinks): Failed to open stream: HTTP request failed! 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Microscale screening systems for 3D cellular microenvironments: platforms, advances, and challenges |pdf=|usr=}}{{25274061 }} gab.com Text Microscale screening systems for 3D cellular microenvironments: platforms, advances, and challenges JO: Cell Mol Life Sci http://www.kidney.de/pget.php?&tw=1&pmid=25274061 #FOAvip The increasing interest in studying cells using more in vivo-like three-dimensional (3D) microenvironments has created a need for advanced 3D screening platforms with enhanced functionalities and increased throughput. 3D screening platforms that better mimic in vivo microenvironments with enhanced throughput would provide more in-depth understanding of the complexity and heterogeneity of microenvironments. The platforms would also better predict the toxicity and efficacy of potential drugs in physiologically relevant conditions. Traditional 3D culture models (e.g., spinner flasks, gyratory rotation devices, non-adhesive surfaces, polymers) were developed to create 3D multicellular structures. However, these traditional systems require large volumes of reagents and cells, and are not compatible with high-throughput screening (HTS) systems. Microscale technology offers the miniaturization of 3D cultures and allows efficient screening of various conditions. This review will discuss the development, most influential works, and current advantages and challenges of microscale culture systems for screening cells in 3D microenvironments. Twit Text FOAVip Microscale screening systems for 3D cellular microenvironments: platforms, advances, and challenges ????Cell Mol Life Sci http://www.kidney.de/pget.php?&tw=1&pmid=25274061 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25274061 #FOAvip English Wikipedia <ref>{{Cite pmid|25274061 }}</ref> Microscale screening systems for 3D cellular microenvironments: platforms, advances, and challenges #MMPMID25274061 Montanez-Sauri SI ; Beebe DJ ; Sung KE Cell Mol Life Sci 2015[Jan]; 72 (2 ): 237-49 PMID25274061 show ga The increasing interest in studying cells using more in vivo-like three-dimensional (3D) microenvironments has created a need for advanced 3D screening platforms with enhanced functionalities and increased throughput. 3D screening platforms that better mimic in vivo microenvironments with enhanced throughput would provide more in-depth understanding of the complexity and heterogeneity of microenvironments. The platforms would also better predict the toxicity and efficacy of potential drugs in physiologically relevant conditions. Traditional 3D culture models (e.g., spinner flasks, gyratory rotation devices, non-adhesive surfaces, polymers) were developed to create 3D multicellular structures. However, these traditional systems require large volumes of reagents and cells, and are not compatible with high-throughput screening (HTS) systems. Microscale technology offers the miniaturization of 3D cultures and allows efficient screening of various conditions. This review will discuss the development, most influential works, and current advantages and challenges of microscale culture systems for screening cells in 3D microenvironments. |Cell Culture Techniques/*methods [MESH] |Cellular Microenvironment/*physiology [MESH] |High-Throughput Screening Assays/*methods [MESH] |Microfluidic Analytical Techniques/*methods [MESH] |Microtechnology/*methods [MESH]Microscale screening systems for 3D cellular microenvironments: platfo(epmc).pdf DeepDyve Pubget Overpricing