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10.1152/ajpcell.00086.2015 http://scihub22266oqcxt.onion/10.1152/ajpcell.00086.2015 C4628935!4628935!26333597     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Physiol+Cell+Physiol 2015 ; 309 (9): C616-26 Nephropedia Template TP {{tp|p=26333597|t=2015. TGF-?1 epigenetically modifies Thy-1 expression in primary lung fibroblasts |pdf=|usr=}}{{26333597}} gab.com Text TGF- 1 epigenetically modifies Thy-1 expression in primary lung fibroblasts JO: Am J Physiol Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=26333597 #FOAvip Idiopathic pulmonary fibrosis is a progressive lung disease that increases in incidence with age. We identified a profibrotic lung phenotype in aging mice characterized by an increase in the number of fibroblasts lacking the expression of thymocyte differentiation antigen 1 (Thy-1) and an increase in transforming growth factor (TGF)-?1 expression. It has been shown that Thy-1 expression can be epigenetically modified. Lung fibroblasts (PLFs) were treated with TGF-?1 ± DNA methyltransferase (DNMT) inhibitor 5-aza-2?-deoxycytidine (5-AZA) and analyzed for Thy-1 gene and protein expression, DNMT protein expression, and activity. ?-Smooth muscle actin (?-SMA) and collagen type 1 (Col1A1) gene and protein expression was assessed. PLFs were transfected with DNMT1 silencing RNA ± TGF-?1. TGF-?1 inhibited Thy-1 gene and protein expression in PLFs, and cotreatment with 5-AZA ameliorated this effect and appeared to inhibit DNMT1 activation. TGF-?1 induced Thy-1 promoter methylation as assessed by quantitative methyl PCR. Treatment with 5-AZA attenuated TGF-?1-induced Col1A1 gene and protein expression and ?-SMA gene expression (but not ?-SMA protein expression). Inhibiting DNMT1 with silencing RNA attenuated TGF-?1-induced DNMT activity and its downstream suppression of Thy-1 mRNA and protein expression as well as inhibited ?-SMA mRNA and Col1A1 mRNA and protein expression, and showed a decreased trend in Thy-1 promoter methylation. Immunofluorescence for ?-SMA suggested that 5-AZA inhibited stress fiber formation. These findings suggest that TGF-?1 epigenetically regulates lung fibroblast phenotype through methylation of the Thy-1 promoter. Targeted inhibition of DNMT in the right clinical context might prevent fibroblast to myofibroblast transdifferentiation and collagen deposition, which in turn could prevent fibrogenesis in the lung and other organs. Twit Text FOAVip TGF- 1 epigenetically modifies Thy-1 expression in primary lung fibroblasts ????Am J Physiol Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=26333597 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26333597 #FOAvip English Wikipedia <ref>{{Cite pmid|26333597}}</ref> TGF-?1 epigenetically modifies Thy-1 expression in primary lung fibroblasts #MMPMID26333597 Neveu WA; Mills ST; Staitieh BS; Sueblinvong V Am J Physiol Cell Physiol 2015[Nov]; 309 (9): C616-26 PMID26333597show ga Idiopathic pulmonary fibrosis is a progressive lung disease that increases in incidence with age. We identified a profibrotic lung phenotype in aging mice characterized by an increase in the number of fibroblasts lacking the expression of thymocyte differentiation antigen 1 (Thy-1) and an increase in transforming growth factor (TGF)-?1 expression. It has been shown that Thy-1 expression can be epigenetically modified. Lung fibroblasts (PLFs) were treated with TGF-?1 ± DNA methyltransferase (DNMT) inhibitor 5-aza-2?-deoxycytidine (5-AZA) and analyzed for Thy-1 gene and protein expression, DNMT protein expression, and activity. ?-Smooth muscle actin (?-SMA) and collagen type 1 (Col1A1) gene and protein expression was assessed. PLFs were transfected with DNMT1 silencing RNA ± TGF-?1. TGF-?1 inhibited Thy-1 gene and protein expression in PLFs, and cotreatment with 5-AZA ameliorated this effect and appeared to inhibit DNMT1 activation. TGF-?1 induced Thy-1 promoter methylation as assessed by quantitative methyl PCR. Treatment with 5-AZA attenuated TGF-?1-induced Col1A1 gene and protein expression and ?-SMA gene expression (but not ?-SMA protein expression). Inhibiting DNMT1 with silencing RNA attenuated TGF-?1-induced DNMT activity and its downstream suppression of Thy-1 mRNA and protein expression as well as inhibited ?-SMA mRNA and Col1A1 mRNA and protein expression, and showed a decreased trend in Thy-1 promoter methylation. Immunofluorescence for ?-SMA suggested that 5-AZA inhibited stress fiber formation. These findings suggest that TGF-?1 epigenetically regulates lung fibroblast phenotype through methylation of the Thy-1 promoter. Targeted inhibition of DNMT in the right clinical context might prevent fibroblast to myofibroblast transdifferentiation and collagen deposition, which in turn could prevent fibrogenesis in the lung and other organs. äTGF-?1 epigenetically modifies Thy-1 expression in primary lung fibrob(epmc).pdf DeepDyve Pubget Overpricing