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10.1039/C5AY00264H http://scihub22266oqcxt.onion/10.1039/C5AY00264H C4627716!4627716!26523155     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Methods 2015 ; 7 (19): 8472-7 Nephropedia Template TP {{tp|p=26523155|t=2015. Development of an automated on-chip bead-based ELISA platform |pdf=|usr=}}{{26523155}} gab.com Text Development of an automated on-chip bead-based ELISA platform JO: Anal Methods http://www.kidney.de/pget.php?&tw=1&pmid=26523155 #FOAvip We present a lab-on-a-chip and associated instrument for heterogeneous enzyme-linked immunosorbent assay (ELISA)-based detection of proteins from liquid samples. The system performs all necessary ELISA steps (starting from antigen incubation) in a quarter of the time required for corresponding plate-based protocols. We have previously described the instrument, which automates fluidic control via remote valve switching and detects fluorescence from reacted substrate, for use in a molecular diagnostics application. The ELISA chip reported here utilizes a high surface area bead bed to enhance capture efficiency and increase the dynamic range of the assay as compared to a standard plate-based ELISA. Its functionality is demonstrated using human IL-10 as a model antigen, but theoretically any sandwich ELISA could be ported onto this ?open source platform.? We show that our automated on-chip assays have greater sensitivities than the corresponding standard manual plate-based ELISAs, and that single samples can be assayed in a fraction of the time. Twit Text FOAVip Development of an automated on-chip bead-based ELISA platform ????Anal Methods http://www.kidney.de/pget.php?&tw=1&pmid=26523155 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26523155 #FOAvip English Wikipedia <ref>{{Cite pmid|26523155}}</ref> Development of an automated on-chip bead-based ELISA platform #MMPMID26523155 Campbell J; Pollock N; Sharon A; Sauer-Budge AF Anal Methods 2015[Oct]; 7 (19): 8472-7 PMID26523155show ga We present a lab-on-a-chip and associated instrument for heterogeneous enzyme-linked immunosorbent assay (ELISA)-based detection of proteins from liquid samples. The system performs all necessary ELISA steps (starting from antigen incubation) in a quarter of the time required for corresponding plate-based protocols. We have previously described the instrument, which automates fluidic control via remote valve switching and detects fluorescence from reacted substrate, for use in a molecular diagnostics application. The ELISA chip reported here utilizes a high surface area bead bed to enhance capture efficiency and increase the dynamic range of the assay as compared to a standard plate-based ELISA. Its functionality is demonstrated using human IL-10 as a model antigen, but theoretically any sandwich ELISA could be ported onto this ?open source platform.? We show that our automated on-chip assays have greater sensitivities than the corresponding standard manual plate-based ELISAs, and that single samples can be assayed in a fraction of the time. äDevelopment of an automated on-chip bead-based ELISA platform (epmc).pdf DeepDyve Pubget Overpricing