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10.1093/nar/gkv964 http://scihub22266oqcxt.onion/10.1093/nar/gkv964 C4627093!4627093 !26433227     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26433227 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nucleic+Acids+Res 2015 ; 43 (19 ): 9350-61 Nephropedia Template TP {{tp|p=26433227 |t=2015. A cytoplasmic pathway for gapmer antisense oligonucleotide-mediated gene silencing in mammalian cells |pdf=|usr=}}{{26433227 }} gab.com Text A cytoplasmic pathway for gapmer antisense oligonucleotide-mediated gene silencing in mammalian cells JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=26433227 #FOAvip Antisense oligonucleotides (ASOs) are known to trigger mRNA degradation in the nucleus via an RNase H-dependent mechanism. We have now identified a putative cytoplasmic mechanism through which ASO gapmers silence their targets when transfected or delivered gymnotically (i.e. in the absence of any transfection reagent). We have shown that the ASO gapmers can interact with the Ago-2 PAZ domain and can localize into GW-182 mRNA-degradation bodies (GW-bodies). The degradation products of the targeted mRNA, however, are not generated by Ago-2-directed cleavage. The apparent identification of a cytoplasmic pathway complements the previously known nuclear activity of ASOs and concurrently suggests that nuclear localization is not an absolute requirement for gene silencing. Twit Text FOAVip A cytoplasmic pathway for gapmer antisense oligonucleotide-mediated gene silencing in mammalian cells ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=26433227 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26433227 #FOAvip English Wikipedia <ref>{{Cite pmid|26433227 }}</ref> A cytoplasmic pathway for gapmer antisense oligonucleotide-mediated gene silencing in mammalian cells #MMPMID26433227 Castanotto D ; Lin M ; Kowolik C ; Wang L ; Ren XQ ; Soifer HS ; Koch T ; Hansen BR ; Oerum H ; Armstrong B ; Wang Z ; Bauer P ; Rossi J ; Stein CA Nucleic Acids Res 2015[Oct]; 43 (19 ): 9350-61 PMID26433227 show ga Antisense oligonucleotides (ASOs) are known to trigger mRNA degradation in the nucleus via an RNase H-dependent mechanism. We have now identified a putative cytoplasmic mechanism through which ASO gapmers silence their targets when transfected or delivered gymnotically (i.e. in the absence of any transfection reagent). We have shown that the ASO gapmers can interact with the Ago-2 PAZ domain and can localize into GW-182 mRNA-degradation bodies (GW-bodies). The degradation products of the targeted mRNA, however, are not generated by Ago-2-directed cleavage. The apparent identification of a cytoplasmic pathway complements the previously known nuclear activity of ASOs and concurrently suggests that nuclear localization is not an absolute requirement for gene silencing. |*Gene Silencing [MESH] |*Oligonucleotides, Antisense/analysis/chemistry/metabolism [MESH] |Argonaute Proteins/metabolism [MESH] |Cell Line [MESH] |Cytoplasm/chemistry/*metabolism [MESH] |Gene Transfer Techniques [MESH] |RNA, Messenger/metabolism [MESH] |RNA, Small Interfering [MESH]A cytoplasmic pathway for gapmer antisense oligonucleotide-mediated ge(epmc).pdf DeepDyve Pubget Overpricing