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2015 ; 10
(5
): e1019983
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English Wikipedia
Importance of phosphoinositide-dependent signaling pathways in the control of
gene expression in resting cells and in response to phytohormones
#MMPMID26039482
Kalachova T
; Kravets V
; Zachowski A
; Ruelland E
Plant Signal Behav
2015[]; 10
(5
): e1019983
PMID26039482
show ga
"Phosphoinositide" refers to phosphorylated forms of phosphatidylinositol,
including phosphatidylinositol-4-phosphate and
phosphatidylinositol-4,5-bisphosphate. Both of these molecules could be in vivo
substrates of plant phospholipase C. These phosphoinositides can also be
biologically active "per se," by directly binding to proteins and thus altering
their location and/or activity. The use of pharmacological agents in Arabidopsis
suspension cells allowed us to identify genes whose expression was positively or
negatively controlled, in the basal state, by products of
phosphoinositide-dependent phospholipase C. In this basal state, it seems that no
genes exhibit a phosphoinositide-dependent expression "per se." However, many
genes whose expression is altered in the presence of phospholipase C inhibitors
appeared to be responsive to salicylic acid. This allowed us to show that
salicylic acid acts both by increasing the phosphoinositide pool and by
inhibiting the phospholipase C. In response to salicylic acid it is possible to
identify genes whose expression is controlled by products of PI-PLC, but also
genes whose expression is controlled by phosphoinositides "per se." Our data
highlight the importance of phosphoinositide-dependent pathways in gene
expression in resting cells and in response to phytohormones.