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10.4161/19420862.2015.989023 http://scihub22266oqcxt.onion/10.4161/19420862.2015.989023 C4622660!4622660!25524068     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       MAbs 2015 ; 7 (1): 180-91 Nephropedia Template TP {{tp|p=25524068|t=2015. Functional inhibition of ?-catenin-mediatedWnt signaling by intracellular VHHantibodies |pdf=|usr=}}{{25524068}} gab.com Text Functional inhibition of -catenin-mediatedWnt signaling by intracellular VHHantibodies JO: MAbs http://www.kidney.de/pget.php?&tw=1&pmid=25524068 #FOAvip The Wnt signaling pathway is of central importance in embryogenesis, development and adult tissue homeostasis, and dysregulation of this pathway is associated with cancer and other diseases. Despite the developmental and potential therapeutic significance of this pathway, many aspects of Wnt signaling, including the control of the master transcriptional co-activator ?-catenin, remain poorly understood. In order to explore this aspect, a diverse immune llama VHH phagemid library was constructed and panned against ?-catenin. VHH antibody fragments from the library were expressed intracellularly, and a number of antibodies were shown to possess function-modifying intracellular activity in a luciferase-based Wnt signaling HEK293 reporter bioassay. Further characterization of one such VHH (named LL3) confirmed that it bound endogenous ?-catenin, and that it inhibited the Wnt signaling pathway downstream of the destruction complex, while production of a control Ala-substituted complementarity-determining region (CDR)3 mutant demonstrated that the inhibition of ?-catenin activity by the parent intracellular antibody was dependent on the specific CDR sequence of the antibody. Twit Text FOAVip Functional inhibition of -catenin-mediatedWnt signaling by intracellular VHHantibodies ????MAbs http://www.kidney.de/pget.php?&tw=1&pmid=25524068 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25524068 #FOAvip English Wikipedia <ref>{{Cite pmid|25524068}}</ref> Functional inhibition of ?-catenin-mediatedWnt signaling by intracellular VHHantibodies #MMPMID25524068 Newnham LE; Wright MJ; Holdsworth G; Kostarelos K; Robinson MK; Rabbitts TH; Lawson AD MAbs 2015[Jan]; 7 (1): 180-91 PMID25524068show ga The Wnt signaling pathway is of central importance in embryogenesis, development and adult tissue homeostasis, and dysregulation of this pathway is associated with cancer and other diseases. Despite the developmental and potential therapeutic significance of this pathway, many aspects of Wnt signaling, including the control of the master transcriptional co-activator ?-catenin, remain poorly understood. In order to explore this aspect, a diverse immune llama VHH phagemid library was constructed and panned against ?-catenin. VHH antibody fragments from the library were expressed intracellularly, and a number of antibodies were shown to possess function-modifying intracellular activity in a luciferase-based Wnt signaling HEK293 reporter bioassay. Further characterization of one such VHH (named LL3) confirmed that it bound endogenous ?-catenin, and that it inhibited the Wnt signaling pathway downstream of the destruction complex, while production of a control Ala-substituted complementarity-determining region (CDR)3 mutant demonstrated that the inhibition of ?-catenin activity by the parent intracellular antibody was dependent on the specific CDR sequence of the antibody. äFunctional inhibition of ?-catenin-mediatedWnt signaling by intracellu(epmc).pdf DeepDyve Pubget Overpricing