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10.4161/19420862.2015.989034 http://scihub22266oqcxt.onion/10.4161/19420862.2015.989034 C4622613!4622613!25524379     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       MAbs 2015 ; 7 (1): 26-31 Nephropedia Template TP {{tp|p=25524379|t=2015. Large scale production of phage antibody libraries using a bioreactor |pdf=|usr=}}{{25524379}} gab.com Text Large scale production of phage antibody libraries using a bioreactor JO: MAbs http://www.kidney.de/pget.php?&tw=1&pmid=25524379 #FOAvip One of the limitations of the use of phage antibody libraries in high throughput selections is the production of sufficient phage antibody library at the appropriate quality. Here, we successfully adapt a bioreactor-based protocol for the production of phage peptide libraries to the production of phage antibody libraries. The titers obtained in the stirred-tank bioreactor are 4 to 5 times higher than in a standard shake flask procedure, and the quality of the phage antibody library produced is indistinguishable to that produced using standard procedures as assessed by Western blotting and functional selections. Availability of this protocol will facilitate the use of phage antibody libraries in high-throughput scale selections. Twit Text FOAVip Large scale production of phage antibody libraries using a bioreactor ????MAbs http://www.kidney.de/pget.php?&tw=1&pmid=25524379 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25524379 #FOAvip English Wikipedia <ref>{{Cite pmid|25524379}}</ref> Large scale production of phage antibody libraries using a bioreactor #MMPMID25524379 Ferrara F; Kim CY; Naranjo LA; Bradbury AR MAbs 2015[Jan]; 7 (1): 26-31 PMID25524379show ga One of the limitations of the use of phage antibody libraries in high throughput selections is the production of sufficient phage antibody library at the appropriate quality. Here, we successfully adapt a bioreactor-based protocol for the production of phage peptide libraries to the production of phage antibody libraries. The titers obtained in the stirred-tank bioreactor are 4 to 5 times higher than in a standard shake flask procedure, and the quality of the phage antibody library produced is indistinguishable to that produced using standard procedures as assessed by Western blotting and functional selections. Availability of this protocol will facilitate the use of phage antibody libraries in high-throughput scale selections. äLarge scale production of phage antibody libraries using a bioreactor (epmc).pdf DeepDyve Pubget Overpricing