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10.1080/15592324.2015.1013793

http://scihub22266oqcxt.onion/10.1080/15592324.2015.1013793
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suck abstract from ncbi


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pmid26039472      Plant+Signal+Behav 2015 ; 10 (5): ä
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  • Chloroplast-generated ROS dominate NaCl- induced K+ efflux in wheat leaf mesophyll #MMPMID26039472
  • Wu H; Shabala L; Zhou M; Shabala S
  • Plant Signal Behav 2015[May]; 10 (5): ä PMID26039472show ga
  • Mesophyll K+ retention ability has been recently reported as an important component of salinity stress tolerance in wheat. In order to investigate the role of ROS in regulating NaCl-induced K+ efflux in wheat leaf mesophyll, a series of pharmacological experiments was conducted using MV (methyl viologen, superoxide radical inducer), DPI (an inhibitor of NADPH oxidase), H2O2 (to mimic apoplastic ROS), and EGCG ((?)-Epigallocatechin gallate, ROS scavenger). Mesophyll pre-treatment with 10 ?M MV resulted in a significantly higher NaCl-induced K+ efflux in leaf mesophyll, while 50 ?M EGCG pre-treatment alleviated K+ leakage under salt stress. No significant change in NaCl-induced K+ efflux in leaf mesophyll was found in specimens pre-treated by H2O2 and DPI, compared with the control. The highest NaCl-induced H+ efflux in leaf mesophyll was also found in samples pre-treated with MV, suggesting a futile cycle between increased H+-ATPase activity and ROS-induced K+ leak. Overall, it is suggested that, under saline stress, K+ efflux from wheat mesophyll is mediated predominantly by non-selective cation channels (NSCC) regulated by ROS produced in chloroplasts, at least in bread wheat.
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