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10.1128/JVI.01781-15

http://scihub22266oqcxt.onion/10.1128/JVI.01781-15
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C4621133!4621133!26311870
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suck abstract from ncbi


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pmid26311870      J+Virol 2015 ; 89 (21): 11169-73
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  • Knockout of cGAS and STING Rescues Virus Infection of Plasmid DNA-Transfected Cells #MMPMID26311870
  • Langereis MA; Rabouw HH; Holwerda M; Visser LJ; van Kuppeveld FJM
  • J Virol 2015[Nov]; 89 (21): 11169-73 PMID26311870show ga
  • It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system, we generated cGAS/STING-knockout cells and show that these cells can be infected after plasmid DNA transfection as efficiently as nontransfected cells.
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