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Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 J+Virol 2015 ; 89 (21): 11169-73 Nephropedia Template TP
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Knockout of cGAS and STING Rescues Virus Infection of Plasmid DNA-Transfected Cells #MMPMID26311870
Langereis MA; Rabouw HH; Holwerda M; Visser LJ; van Kuppeveld FJM
J Virol 2015[Nov]; 89 (21): 11169-73 PMID26311870show ga
It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system, we generated cGAS/STING-knockout cells and show that these cells can be infected after plasmid DNA transfection as efficiently as nontransfected cells.