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10.1186/s12864-015-2097-9 http://scihub22266oqcxt.onion/10.1186/s12864-015-2097-9 C4619272!4619272!26493562     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Genomics 2015 ; 16 (ä): ä Nephropedia Template TP {{tp|p=26493562|t=2015. Novel method to detect microRNAs using chip-based QuantStudio 3D digital PCR |pdf=|usr=}}{{26493562}} gab.com Text Novel method to detect microRNAs using chip-based QuantStudio 3D digital PCR JO: BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=26493562 #FOAvip Background: Research efforts for the management of cancer, in particular for lung cancer, are directed to identify new strategies for its early detection. MicroRNAs (miRNAs) are a new promising class of circulating biomarkers for cancer detection, but lack of consensus on data normalization methods has affected the diagnostic potential of circulating miRNAs. There is a growing interest in techniques that allow an absolute quantification of miRNAs which could be useful for early diagnosis. Recently, digital PCR, mainly based on droplets generation, emerged as an affordable technology for precise and absolute quantification of nucleic acids. Results: In this work, we described a new interesting approach for profiling circulating miRNAs in plasma samples using a chip-based platform, the QuantStudio 3D digital PCR. The proposed method was validated using synthethic oligonucleotide at serial dilutions in plasma samples of lung cancer patients and in lung tissues and cell lines. Conclusion: Given its reproducibility and reliability, our approach could be potentially applied for the identification and quantification of miRNAs in other biological samples such as circulating exosomes or protein complexes. As chip-digital PCR becomes more established, it would be a robust tool for quantitative assessment of miRNA copy number for diagnosis of lung cancer and other diseases. Electronic supplementary material: The online version of this article (doi:10.1186/s12864-015-2097-9) contains supplementary material, which is available to authorized users. Twit Text FOAVip Novel method to detect microRNAs using chip-based QuantStudio 3D digital PCR ????BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=26493562 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26493562 #FOAvip English Wikipedia <ref>{{Cite pmid|26493562}}</ref> Novel method to detect microRNAs using chip-based QuantStudio 3D digital PCR #MMPMID26493562 Conte D; Verri C; Borzi C; Suatoni P; Pastorino U; Sozzi G; Fortunato O BMC Genomics 2015[]; 16 (ä): ä PMID26493562show ga Background: Research efforts for the management of cancer, in particular for lung cancer, are directed to identify new strategies for its early detection. MicroRNAs (miRNAs) are a new promising class of circulating biomarkers for cancer detection, but lack of consensus on data normalization methods has affected the diagnostic potential of circulating miRNAs. There is a growing interest in techniques that allow an absolute quantification of miRNAs which could be useful for early diagnosis. Recently, digital PCR, mainly based on droplets generation, emerged as an affordable technology for precise and absolute quantification of nucleic acids. Results: In this work, we described a new interesting approach for profiling circulating miRNAs in plasma samples using a chip-based platform, the QuantStudio 3D digital PCR. The proposed method was validated using synthethic oligonucleotide at serial dilutions in plasma samples of lung cancer patients and in lung tissues and cell lines. Conclusion: Given its reproducibility and reliability, our approach could be potentially applied for the identification and quantification of miRNAs in other biological samples such as circulating exosomes or protein complexes. As chip-digital PCR becomes more established, it would be a robust tool for quantitative assessment of miRNA copy number for diagnosis of lung cancer and other diseases. Electronic supplementary material: The online version of this article (doi:10.1186/s12864-015-2097-9) contains supplementary material, which is available to authorized users. äNovel method to detect microRNAs using chip-based QuantStudio 3D digit(epmc).pdf DeepDyve Pubget Overpricing