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10.1097/TP.0000000000000762 http://scihub22266oqcxt.onion/10.1097/TP.0000000000000762 C4618723!4618723 !25989500     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25989500 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Transplantation 2015 ; 99 (11 ): 2274-84 Nephropedia Template TP {{tp|p=25989500 |t=2015. Stimulation of Dopamine D3 Receptor Attenuates Renal Ischemia-Reperfusion Injury via Increased Linkage With G?12 |pdf=|usr=}}{{25989500 }} gab.com Text Stimulation of Dopamine D3 Receptor Attenuates Renal Ischemia-Reperfusion Injury via Increased Linkage With G 12 JO: Transplantation http://www.kidney.de/pget.php?&tw=1&pmid=25989500 #FOAvip BACKGROUND: Renal ischemia-reperfusion (I/R) injury causes renal tubular necrosis, apoptosis, and inflammation leading to acute renal dysfunction. Recent studies have revealed that deletion of G?12 mitigates the renal damage due to I/R injury. Our previous study showed that activation of dopamine D3 receptor (D3R) increased its linkage with G?12, and hampered G?12-mediated stimulation of renal sodium transport. In the present study, we used an in-vivo rat model and an in vitro study of the renal epithelial cell line (NRK52E) to investigate whether or not an increased linkage between D3R and G?12 contributes to the protective effect of D3R on renal I/R injury. METHODS: For in vivo studies, I/R injury was induced in a rat renal unilateral clamping model. For in vitro studies, hypoxia/reoxygenation and cold storage/rewarming injuries were performed in NRK52E cells. PD128907, a D3R agonist, or vehicle, was administered 15 minutes before clamping (or hypoxia) in both the in vivo or in vitro studies. RESULTS: In the rat renal unilateral clamping model, pretreatment with PD128907 (0.2 mg/kg, intravenous) protected against renal I/R injury and increased survival rate during a long-term follow-up after 7 days. A decrease in the generation of reactive oxygen species, apoptosis, and inflammation may be involved in the D3R-mediated protection because pretreatment with PD128907 increased renal glutathione and superoxide dismutase levels and decreased malondialdehyde levels in the I/R group. The increase in cytokines (TNF-?, IL-1?, and IL-10) and myeloperoxidase in I/R injured kidney was also prevented with a simultaneous decrease in the apoptosis of the epithelial cells and expression of apoptosis biomarkers in kidney harvested 1 day after I/R injury. The increase in the coimmunoprecipitation between D3R and G?12 with D3R stimulation paralleled the observed renal protection from I/R injury. Moreover, in vitro studies showed that transient overexpression of G?12 in the NRK52E cells attenuated the protective effect of PD128907 on hypoxia/reoxygenation injury. The protective effect of PD128907 might be of significance to renal transplantation because cold storage/rewarming induced injury increased lactate dehydrogenase release and decreased cell viability in NRK52E cells. Conversely, in the presence of PD128907, the increased lactate dehydrogenase release and decreased cell viability were reversed. CONCLUSIONS: These results suggest that activation of D3R, by decreasing G?12-induced renal damage, may exert a protective effect from I/R injury. Twit Text FOAVip Stimulation of Dopamine D3 Receptor Attenuates Renal Ischemia-Reperfusion Injury via Increased Linkage With G 12 ????Transplantation http://www.kidney.de/pget.php?&tw=1&pmid=25989500 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25989500 #FOAvip English Wikipedia <ref>{{Cite pmid|25989500 }}</ref> Stimulation of Dopamine D3 Receptor Attenuates Renal Ischemia-Reperfusion Injury via Increased Linkage With G?12 #MMPMID25989500 Wang Z ; Guan W ; Han Y ; Ren H ; Tang X ; Zhang H ; Liu Y ; Fu J ; He D ; Asico LD ; Jose PA ; Zhou L ; Chen L ; Zeng C Transplantation 2015[Nov]; 99 (11 ): 2274-84 PMID25989500 show ga BACKGROUND: Renal ischemia-reperfusion (I/R) injury causes renal tubular necrosis, apoptosis, and inflammation leading to acute renal dysfunction. Recent studies have revealed that deletion of G?12 mitigates the renal damage due to I/R injury. Our previous study showed that activation of dopamine D3 receptor (D3R) increased its linkage with G?12, and hampered G?12-mediated stimulation of renal sodium transport. In the present study, we used an in-vivo rat model and an in vitro study of the renal epithelial cell line (NRK52E) to investigate whether or not an increased linkage between D3R and G?12 contributes to the protective effect of D3R on renal I/R injury. METHODS: For in vivo studies, I/R injury was induced in a rat renal unilateral clamping model. For in vitro studies, hypoxia/reoxygenation and cold storage/rewarming injuries were performed in NRK52E cells. PD128907, a D3R agonist, or vehicle, was administered 15 minutes before clamping (or hypoxia) in both the in vivo or in vitro studies. RESULTS: In the rat renal unilateral clamping model, pretreatment with PD128907 (0.2 mg/kg, intravenous) protected against renal I/R injury and increased survival rate during a long-term follow-up after 7 days. A decrease in the generation of reactive oxygen species, apoptosis, and inflammation may be involved in the D3R-mediated protection because pretreatment with PD128907 increased renal glutathione and superoxide dismutase levels and decreased malondialdehyde levels in the I/R group. The increase in cytokines (TNF-?, IL-1?, and IL-10) and myeloperoxidase in I/R injured kidney was also prevented with a simultaneous decrease in the apoptosis of the epithelial cells and expression of apoptosis biomarkers in kidney harvested 1 day after I/R injury. The increase in the coimmunoprecipitation between D3R and G?12 with D3R stimulation paralleled the observed renal protection from I/R injury. Moreover, in vitro studies showed that transient overexpression of G?12 in the NRK52E cells attenuated the protective effect of PD128907 on hypoxia/reoxygenation injury. The protective effect of PD128907 might be of significance to renal transplantation because cold storage/rewarming induced injury increased lactate dehydrogenase release and decreased cell viability in NRK52E cells. Conversely, in the presence of PD128907, the increased lactate dehydrogenase release and decreased cell viability were reversed. CONCLUSIONS: These results suggest that activation of D3R, by decreasing G?12-induced renal damage, may exert a protective effect from I/R injury. |Acute Kidney Injury/metabolism/pathology/*prevention & control [MESH] |Animals [MESH] |Apoptosis/drug effects [MESH] |Benzopyrans/*pharmacology [MESH] |Cell Line [MESH] |Cell Survival/drug effects [MESH] |Cytoprotection [MESH] |Disease Models, Animal [MESH] |Dopamine Agonists/*pharmacology [MESH] |Epithelial Cells/drug effects/metabolism/pathology [MESH] |GTP-Binding Protein alpha Subunits, G12-G13/genetics/*metabolism [MESH] |Glutathione/metabolism [MESH] |Inflammation Mediators/metabolism [MESH] |Kidney/blood supply/*drug effects/metabolism/pathology [MESH] |Male [MESH] |Malondialdehyde/metabolism [MESH] |Oxazines/*pharmacology [MESH] |Oxidative Stress/drug effects [MESH] |Rats, Wistar [MESH] |Receptors, Dopamine D3/*agonists/metabolism [MESH] |Reperfusion Injury/metabolism/pathology/*prevention & control [MESH] |Signal Transduction/drug effects [MESH] |Superoxide Dismutase/metabolism [MESH] |Time Factors [MESH]Stimulation of Dopamine D3 Receptor Attenuates Renal Ischemia-Reperfus(epmc).pdf DeepDyve Pubget Overpricing