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10.1038/nbt.3190 http://scihub22266oqcxt.onion/10.1038/nbt.3190 C4618510!4618510 !25798939     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25798939 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Biotechnol 2015 ; 33 (5 ): 538-42 Nephropedia Template TP {{tp|p=25798939 |t=2015. Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining |pdf=|usr=}}{{25798939 }} gab.com Text Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining JO: Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=25798939 #FOAvip Methods to introduce targeted double-strand breaks (DSBs) into DNA enable precise genome editing by increasing the rate at which externally supplied DNA fragments are incorporated into the genome through homologous recombination. The efficiency of these methods is limited by nonhomologous end joining (NHEJ), an alternative DNA repair pathway that competes with homology-directed repair (HDR). To promote HDR at the expense of NHEJ, we targeted DNA ligase IV, a key enzyme in the NHEJ pathway, using the inhibitor Scr7. Scr7 treatment increased the efficiency of HDR-mediated genome editing, using Cas9 in mammalian cell lines and in mice for all four genes examined, up to 19-fold. This approach should be applicable to other customizable endonucleases, such as zinc finger nucleases and transcription activator-like effector nucleases, and to nonmammalian cells with sufficiently conserved mechanisms of NHEJ and HDR. Twit Text FOAVip Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining ????Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=25798939 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25798939 #FOAvip English Wikipedia <ref>{{Cite pmid|25798939 }}</ref> Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining #MMPMID25798939 Maruyama T ; Dougan SK ; Truttmann MC ; Bilate AM ; Ingram JR ; Ploegh HL Nat Biotechnol 2015[May]; 33 (5 ): 538-42 PMID25798939 show ga Methods to introduce targeted double-strand breaks (DSBs) into DNA enable precise genome editing by increasing the rate at which externally supplied DNA fragments are incorporated into the genome through homologous recombination. The efficiency of these methods is limited by nonhomologous end joining (NHEJ), an alternative DNA repair pathway that competes with homology-directed repair (HDR). To promote HDR at the expense of NHEJ, we targeted DNA ligase IV, a key enzyme in the NHEJ pathway, using the inhibitor Scr7. Scr7 treatment increased the efficiency of HDR-mediated genome editing, using Cas9 in mammalian cell lines and in mice for all four genes examined, up to 19-fold. This approach should be applicable to other customizable endonucleases, such as zinc finger nucleases and transcription activator-like effector nucleases, and to nonmammalian cells with sufficiently conserved mechanisms of NHEJ and HDR. |Animals [MESH] |CRISPR-Cas Systems/*genetics [MESH] |Cell Line [MESH] |DNA Breaks, Double-Stranded/drug effects [MESH] |DNA End-Joining Repair/*genetics [MESH] |DNA Ligase ATP [MESH] |DNA Ligases/*genetics [MESH] |DNA Repair/genetics [MESH] |Genetic Engineering/*methods [MESH] |Genome [MESH] |Homologous Recombination/genetics [MESH] |Mice [MESH] |Pyrimidines/administration & dosage [MESH] |Schiff Bases/administration & dosage [MESH]Increasing the efficiency of precise genome editing with CRISPR-Cas9 b(epmc).pdf DeepDyve Pubget Overpricing