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10.1194/jlr.M045583

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suck abstract from ncbi


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pmid25187667
      J+Lipid+Res 2014 ; 55 (11 ): 2401-7
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  • Short-term n-3 fatty acid supplementation but not aspirin increases plasma proresolving mediators of inflammation #MMPMID25187667
  • Barden A ; Mas E ; Croft KD ; Phillips M ; Mori TA
  • J Lipid Res 2014[Nov]; 55 (11 ): 2401-7 PMID25187667 show ga
  • Resolution of inflammation is an active process involving specialized proresolving mediators (SPM) formed from the n-3 fatty acids. This study examined the effect of n-3 fatty acid supplementation and aspirin on plasma SPMs in healthy humans. Healthy volunteers (n = 21) were supplemented with n-3 fatty acids (2.4g/day) for 7 days with random assignment to take aspirin (300 mg/day) or placebo from day 5 to day 7. Blood was collected at baseline (day 0), day 5, and day 7. Plasma 18R/S-HEPE, E-series resolvins, 17R/S-HDHA, D-series resolvins, 14R/S-HDHA, and MaR-1 were measured by LC/MS/MS. At baseline concentrations of E- and D- series resolvins and the upstream precursors 18R/S-HEPE, 17R/S-HDHA ranged from 0.1nM to 0.2nM. 14R/S-HDHA was 3-fold higher than the other SPMs at baseline but MaR-1 was below the limit of detection. Supplementation with n-3 fatty acids significantly increased RvE1, 18R/S-HEPE, 17R/S-HDHA, and 14R/S-HDHA but not other SPMs. The addition of aspirin after 5 days of n-3 fatty acids did not affect concentrations of any SPM. N-3 fatty acid supplementation for 5 days results in concentrations of SPMs that are biologically active in healthy humans. Aspirin administered after n-3 fatty acids did not offer any additional benefit in elevating the levels of SPMs.
  • |Adult [MESH]
  • |Aged [MESH]
  • |Aspirin/*pharmacology [MESH]
  • |Fatty Acids, Omega-3/chemistry/*pharmacology [MESH]
  • |Female [MESH]
  • |Humans [MESH]
  • |Inflammation Mediators/*blood [MESH]
  • |Isomerism [MESH]
  • |Male [MESH]


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