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10.1194/jlr.M051821 http://scihub22266oqcxt.onion/10.1194/jlr.M051821 C4617136!4617136 !25274613     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25274613 &cmd=llinks): Failed to open stream: HTTP request failed! 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Quantitative imaging mass spectrometry of renal sulfatides: validation by classical mass spectrometric methods |pdf=|usr=}}{{25274613 }} gab.com Text Quantitative imaging mass spectrometry of renal sulfatides: validation by classical mass spectrometric methods JO: J Lipid Res http://www.kidney.de/pget.php?&tw=1&pmid=25274613 #FOAvip Owing to its capability of discriminating subtle mass-altering structural differences such as double bonds or elongated acyl chains, MALDI-based imaging MS (IMS) has emerged as a powerful technique for analysis of lipid distribution in tissue at moderate spatial resolution of about 50 ?m. However, it is still unknown if MS(1)-signals and ion intensity images correlate with the corresponding apparent lipid concentrations. Analyzing renal sulfated glycosphingolipids, sulfatides, we validate for the first time IMS-signal identities using corresponding sulfatide-deficient kidneys. To evaluate the extent of signal quenching effects interfering with lipid quantification, we surgically dissected the three major renal regions (papillae, medulla, and cortex) and systematically compared MALDI IMS of renal sulfatides with quantitative analyses of corresponding lipid extracts by on-target MALDI TOF-MS and by ultra-performance LC-ESI-(triple-quadrupole)tandem MS. Our results demonstrate a generally strong correlation (R(2) > 0.9) between the local relative sulfatide signal intensity in MALDI IMS and absolute sulfatide quantities determined by the other two methods. However, high concentrations of sulfatides in the papillae and medulla result in an up to 4-fold signal suppression. In conclusion, our study suggests that MALDI IMS is useful for semi-quantitative dissection of relative local changes of sulfatides and possibly other lipids in tissue. Twit Text FOAVip Quantitative imaging mass spectrometry of renal sulfatides: validation by classical mass spectrometric methods ????J Lipid Res http://www.kidney.de/pget.php?&tw=1&pmid=25274613 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25274613 #FOAvip English Wikipedia <ref>{{Cite pmid|25274613 }}</ref> Quantitative imaging mass spectrometry of renal sulfatides: validation by classical mass spectrometric methods #MMPMID25274613 Marsching C ; Jennemann R ; Heilig R ; Gröne HJ ; Hopf C ; Sandhoff R J Lipid Res 2014[Nov]; 55 (11 ): 2343-53 PMID25274613 show ga Owing to its capability of discriminating subtle mass-altering structural differences such as double bonds or elongated acyl chains, MALDI-based imaging MS (IMS) has emerged as a powerful technique for analysis of lipid distribution in tissue at moderate spatial resolution of about 50 ?m. However, it is still unknown if MS(1)-signals and ion intensity images correlate with the corresponding apparent lipid concentrations. Analyzing renal sulfated glycosphingolipids, sulfatides, we validate for the first time IMS-signal identities using corresponding sulfatide-deficient kidneys. To evaluate the extent of signal quenching effects interfering with lipid quantification, we surgically dissected the three major renal regions (papillae, medulla, and cortex) and systematically compared MALDI IMS of renal sulfatides with quantitative analyses of corresponding lipid extracts by on-target MALDI TOF-MS and by ultra-performance LC-ESI-(triple-quadrupole)tandem MS. Our results demonstrate a generally strong correlation (R(2) > 0.9) between the local relative sulfatide signal intensity in MALDI IMS and absolute sulfatide quantities determined by the other two methods. However, high concentrations of sulfatides in the papillae and medulla result in an up to 4-fold signal suppression. In conclusion, our study suggests that MALDI IMS is useful for semi-quantitative dissection of relative local changes of sulfatides and possibly other lipids in tissue. |Animals [MESH] |Female [MESH] |Kidney/*metabolism [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Molecular Imaging/*methods [MESH] |Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods [MESH]Quantitative imaging mass spectrometry of renal sulfatides: validation(epmc).pdf DeepDyve Pubget Overpricing