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10.4161/rna.29947 http://scihub22266oqcxt.onion/10.4161/rna.29947 C4615833!4615833!25482893     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       RNA+Biol 2014 ; 11 (8): 986-91 Nephropedia Template TP {{tp|p=25482893|t=2014. A single molecule approach to mRNA transport by a class V myosin |pdf=|usr=}}{{25482893}} gab.com Text A single molecule approach to mRNA transport by a class V myosin JO: RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=25482893 #FOAvip mRNA localization ensures correct spatial and temporal control of protein synthesis in the cell. We show that an in vitro single molecule approach, using purified recombinant full-length proteins and synthesized mRNA, provides insight into the mechanism by which localizing mRNAs are carried to their destination. A messenger ribonucleoprotein (mRNP) complex was reconstituted from a budding yeast class V myosin motor complex (Myo4p?She3p), an mRNA-binding adaptor protein (She2p), and a localizing mRNA (ASH1). The motion of the mRNP was tracked with high spatial (?10 nm) and temporal (70 ms) resolution. Using this ?bottom-up? methodology, we show that mRNA triggers the assembly of a high affinity double-headed motor-mRNA complex that moves continuously for long distances on actin filaments at physiologic ionic strength. Without mRNA, the myosin is monomeric and unable to move continuously on actin. This finding reveals an elegant strategy to ensure that only cargo-bound motors are activated for transport. Increasing the number of localization elements (?zip codes?) in the mRNA enhanced both the frequency of motile events and their run length, features which likely enhance cellular localization. Future in vitro reconstitution of mRNPs with kinesin and dynein motors should similarly yield mechanistic insight into mRNA transport by microtubule-based motors. Twit Text FOAVip A single molecule approach to mRNA transport by a class V myosin ????RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=25482893 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25482893 #FOAvip English Wikipedia <ref>{{Cite pmid|25482893}}</ref> A single molecule approach to mRNA transport by a class V myosin #MMPMID25482893 Sladewski TE; Trybus KM RNA Biol 2014[Aug]; 11 (8): 986-91 PMID25482893show ga mRNA localization ensures correct spatial and temporal control of protein synthesis in the cell. We show that an in vitro single molecule approach, using purified recombinant full-length proteins and synthesized mRNA, provides insight into the mechanism by which localizing mRNAs are carried to their destination. A messenger ribonucleoprotein (mRNP) complex was reconstituted from a budding yeast class V myosin motor complex (Myo4p?She3p), an mRNA-binding adaptor protein (She2p), and a localizing mRNA (ASH1). The motion of the mRNP was tracked with high spatial (?10 nm) and temporal (70 ms) resolution. Using this ?bottom-up? methodology, we show that mRNA triggers the assembly of a high affinity double-headed motor-mRNA complex that moves continuously for long distances on actin filaments at physiologic ionic strength. Without mRNA, the myosin is monomeric and unable to move continuously on actin. This finding reveals an elegant strategy to ensure that only cargo-bound motors are activated for transport. Increasing the number of localization elements (?zip codes?) in the mRNA enhanced both the frequency of motile events and their run length, features which likely enhance cellular localization. Future in vitro reconstitution of mRNPs with kinesin and dynein motors should similarly yield mechanistic insight into mRNA transport by microtubule-based motors. äA single molecule approach to mRNA transport by a class V myosin (epmc).pdf DeepDyve Pubget Overpricing