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Single-molecule tools for enzymology, structural biology, systems biology and
nanotechnology: an update
#MMPMID25212907
Widom JR
; Dhakal S
; Heinicke LA
; Walter NG
Arch Toxicol
2014[Nov]; 88
(11
): 1965-85
PMID25212907
show ga
Toxicology is the highly interdisciplinary field studying the adverse effects of
chemicals on living organisms. It requires sensitive tools to detect such
effects. After their initial implementation during the 1990s, single-molecule
fluorescence detection tools were quickly recognized for their potential to
contribute greatly to many different areas of scientific inquiry. In the
intervening time, technical advances in the field have generated ever-improving
spatial and temporal resolution and have enabled the application of
single-molecule fluorescence to increasingly complex systems, such as live cells.
In this review, we give an overview of the optical components necessary to
implement the most common versions of single-molecule fluorescence detection. We
then discuss current applications to enzymology and structural studies, systems
biology, and nanotechnology, presenting the technical considerations that are
unique to each area of study, along with noteworthy recent results. We also
highlight future directions that have the potential to revolutionize these areas
of study by further exploiting the capabilities of single-molecule fluorescence
microscopy.
free
free
free
