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10.3390/s150924178 http://scihub22266oqcxt.onion/10.3390/s150924178 C4610488!4610488 !26393614     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26393614 &cmd=llinks): Failed to open stream: HTTP request failed! 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Continuous-Wave Stimulated Emission Depletion Microscope for Imaging Actin Cytoskeleton in Fixed and Live Cells |pdf=|usr=}}{{26393614 }} gab.com Text Continuous-Wave Stimulated Emission Depletion Microscope for Imaging Actin Cytoskeleton in Fixed and Live Cells JO: Sensors (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=26393614 #FOAvip Stimulated emission depletion (STED) microscopy provides a new opportunity to study fine sub-cellular structures and highly dynamic cellular processes, which are challenging to observe using conventional optical microscopy. Using actin as an example, we explored the feasibility of using a continuous wave (CW)-STED microscope to study the fine structure and dynamics in fixed and live cells. Actin plays an important role in cellular processes, whose functioning involves dynamic formation and reorganization of fine structures of actin filaments. Frequently used confocal fluorescence and STED microscopy dyes were employed to image fixed PC-12 cells (dyed with phalloidin- fluorescein isothiocyante) and live rat chondrosarcoma cells (RCS) transfected with actin-green fluorescent protein (GFP). Compared to conventional confocal fluorescence microscopy, CW-STED microscopy shows improved spatial resolution in both fixed and live cells. We were able to monitor cell morphology changes continuously; however, the number of repetitive analyses were limited primarily by the dyes used in these experiments and could be improved with the use of dyes less susceptible to photobleaching. In conclusion, CW-STED may disclose new information for biological systems with a proper characteristic length scale. The challenges of using CW-STED microscopy to study cell structures are discussed. Twit Text FOAVip Continuous-Wave Stimulated Emission Depletion Microscope for Imaging Actin Cytoskeleton in Fixed and Live Cells ????Sensors (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=26393614 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26393614 #FOAvip English Wikipedia <ref>{{Cite pmid|26393614 }}</ref> Continuous-Wave Stimulated Emission Depletion Microscope for Imaging Actin Cytoskeleton in Fixed and Live Cells #MMPMID26393614 Neupane B ; Jin T ; Mellor LF ; Loboa EG ; Ligler FS ; Wang G Sensors (Basel) 2015[Sep]; 15 (9 ): 24178-90 PMID26393614 show ga Stimulated emission depletion (STED) microscopy provides a new opportunity to study fine sub-cellular structures and highly dynamic cellular processes, which are challenging to observe using conventional optical microscopy. Using actin as an example, we explored the feasibility of using a continuous wave (CW)-STED microscope to study the fine structure and dynamics in fixed and live cells. Actin plays an important role in cellular processes, whose functioning involves dynamic formation and reorganization of fine structures of actin filaments. Frequently used confocal fluorescence and STED microscopy dyes were employed to image fixed PC-12 cells (dyed with phalloidin- fluorescein isothiocyante) and live rat chondrosarcoma cells (RCS) transfected with actin-green fluorescent protein (GFP). Compared to conventional confocal fluorescence microscopy, CW-STED microscopy shows improved spatial resolution in both fixed and live cells. We were able to monitor cell morphology changes continuously; however, the number of repetitive analyses were limited primarily by the dyes used in these experiments and could be improved with the use of dyes less susceptible to photobleaching. In conclusion, CW-STED may disclose new information for biological systems with a proper characteristic length scale. The challenges of using CW-STED microscopy to study cell structures are discussed. |*Tissue Fixation [MESH] |Actin Cytoskeleton/*metabolism [MESH] |Animals [MESH] |Cell Line, Tumor [MESH] |Cell Survival [MESH] |Chondrocytes/cytology [MESH] |Fluorescence [MESH] |Imaging, Three-Dimensional/*instrumentation [MESH] |Microscopy, Confocal [MESH] |Microscopy/*instrumentation [MESH] |PC12 Cells [MESH]Continuous-Wave Stimulated Emission Depletion Microscope for Imaging A(epmc).pdf DeepDyve Pubget Overpricing