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10.1007/978-1-62703-974-1_17

http://scihub22266oqcxt.onion/10.1007/978-1-62703-974-1_17
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C4609530!4609530!24567220
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suck abstract from ncbi


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pmid24567220      Methods+Mol+Biol 2014 ; 1128 (ä): 249-62
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  • Multicolor Labeling in Developmental Gene Regulatory Network Analysis #MMPMID24567220
  • Sethi AJ; Angerer RC; Angerer LM
  • Methods Mol Biol 2014[]; 1128 (ä): 249-62 PMID24567220show ga
  • The sea urchin embryo is an important model system for developmental gene regulatory network (GRN) analysis. This chapter describes the use of multicolor fluorescent in situ hybridization (FISH) as well as a combination of FISH and immunohistochemistry in sea urchin embryonic GRN studies. The methods presented here can be applied to a variety of experimental settings where accurate spatial resolution of multiple gene products is required for constructing a developmental GRN.
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