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10.1186/s12864-015-2027-x http://scihub22266oqcxt.onion/10.1186/s12864-015-2027-x C4609152!4609152 !26474969     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26474969 &cmd=llinks): Failed to open stream: HTTP request failed! 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Development of genome-wide InDel markers and their integration with SSR, DArT and SNP markers in single barley map |pdf=|usr=}}{{26474969 }} gab.com Text Development of genome-wide InDel markers and their integration with SSR, DArT and SNP markers in single barley map JO: BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=26474969 #FOAvip BACKGROUND: Development of molecular markers such as SSR (simple sequence repeat), DArT (diversity arrays technology) and SNP (single nucleotide polymorphism) is fundamental for linkage map construction and QTL mapping. However, DArT and SNP genotyping require special tools, and detection of SSR polymorphisms requires time-consuming polyacrylamide electrophoresis. Furthermore, many markers have been mapped in different populations such that their genetic positions are inconsistent. Recently, InDel (insertion and deletion) markers have become popular in genetic map construction and map-based cloning. RESULTS: Aligning genomic DNA sequences in two barley cultivars (Morex and Barke) identified 436,640 InDels. We designed 1140 InDel markers across the barley genome with an average genetic distance of 1 cM, each having a unique location in the barley genome. High-resolution melting (HRM) technology was used to genotype 55 InDel markers; those PCR amplicons with melting temperature differences >0.3 °C were ideal for HRM genotyping. The 1140 InDel markers together with 383 SSRs, 3909 gene-based SNPs and 1544 DArT markers were integrated into single barley genetic map according to their physical map positions. CONCLUSIONS: High-density InDel markers with specific genome locations were developed, with 6976 molecular markers (SSRs, DArTs, SNPs and InDels) integrated into single barley genetic map. HRM genotyping of the InDel markers each with single PCR band will facilitate quick map construction and gene fine-mapping. Twit Text FOAVip Development of genome-wide InDel markers and their integration with SSR, DArT and SNP markers in single barley map ????BMC Genomics http://www.kidney.de/pget.php?&tw=1&pmid=26474969 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26474969 #FOAvip English Wikipedia <ref>{{Cite pmid|26474969 }}</ref> Development of genome-wide InDel markers and their integration with SSR, DArT and SNP markers in single barley map #MMPMID26474969 Zhou G ; Zhang Q ; Tan C ; Zhang XQ ; Li C BMC Genomics 2015[Oct]; 16 (ä): 804 PMID26474969 show ga BACKGROUND: Development of molecular markers such as SSR (simple sequence repeat), DArT (diversity arrays technology) and SNP (single nucleotide polymorphism) is fundamental for linkage map construction and QTL mapping. However, DArT and SNP genotyping require special tools, and detection of SSR polymorphisms requires time-consuming polyacrylamide electrophoresis. Furthermore, many markers have been mapped in different populations such that their genetic positions are inconsistent. Recently, InDel (insertion and deletion) markers have become popular in genetic map construction and map-based cloning. RESULTS: Aligning genomic DNA sequences in two barley cultivars (Morex and Barke) identified 436,640 InDels. We designed 1140 InDel markers across the barley genome with an average genetic distance of 1 cM, each having a unique location in the barley genome. High-resolution melting (HRM) technology was used to genotype 55 InDel markers; those PCR amplicons with melting temperature differences >0.3 °C were ideal for HRM genotyping. The 1140 InDel markers together with 383 SSRs, 3909 gene-based SNPs and 1544 DArT markers were integrated into single barley genetic map according to their physical map positions. CONCLUSIONS: High-density InDel markers with specific genome locations were developed, with 6976 molecular markers (SSRs, DArTs, SNPs and InDels) integrated into single barley genetic map. HRM genotyping of the InDel markers each with single PCR band will facilitate quick map construction and gene fine-mapping. |*Genetic Markers [MESH] |Chromosome Mapping [MESH] |Expressed Sequence Tags [MESH] |Genetic Linkage [MESH] |Genome, Plant [MESH] |Hordeum/*genetics [MESH] |INDEL Mutation/*genetics [MESH] |Microsatellite Repeats/*genetics [MESH] |Polymorphism, Single Nucleotide/genetics [MESH]Development of genome-wide InDel markers and their integration with SS(epmc).pdf DeepDyve Pubget Overpricing