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10.1111/cei.12688 http://scihub22266oqcxt.onion/10.1111/cei.12688 C4608512!4608512 !26212048     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26212048 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Clin+Exp+Immunol 2015 ; 182 (2 ): 220-9 Nephropedia Template TP {{tp|p=26212048 |t=2015. Messenger RNA encoding constitutively active Toll-like receptor 4 enhances effector functions of human T cells |pdf=|usr=}}{{26212048 }} gab.com Text Messenger RNA encoding constitutively active Toll-like receptor 4 enhances effector functions of human T cells JO: Clin Exp Immunol http://www.kidney.de/pget.php?&tw=1&pmid=26212048 #FOAvip Adoptive T cell therapy of cancer employs a large number of ex-vivo-propagated T cells which recognize their targets either by virtue of their endogenous T cell receptor (TCR) or via genetic reprogramming. However, both cell-extrinsic and intrinsic mechanisms often diminish the in-vivo potency of these therapeutic T cells, limiting their clinical efficacy and broader use. Direct activation of human T cells by Toll-like receptor (TLR) ligands induces T cell survival and proliferation, boosts the production of proinflammatory cytokines and augments resistance to regulatory T cell (Treg) suppression. Removal of the TLR ligand-binding region results in constitutive signalling triggered by the remaining cytosolic Toll/interleukin-1 receptor (TIR) domain. The use of such TIR domains therefore offers an ideal means for equipping anti-tumour T cells with the arsenal of functional attributes required for improving current clinical protocols. Here we show that constitutively active (ca)TLR-4 can be expressed efficiently in human T cells using mRNA electroporation. The mere expression of caTLR-4 mRNA in polyclonal CD8 and CD4 T cells induced the production of interferon (IFN)-?, triggered the surface expression of CD25, CD69 and 4-1BB and up-regulated a panel of cytokines and chemokines. In tumour-infiltrating lymphocytes prepared from melanoma patients, caTLR-4 induced robust IFN-? secretion in all samples tested. Furthermore, caTLR-4 enhanced the anti-melanoma cytolytic activity of tumour-infiltrating lymphocytes and augmented the secretion of IFN-?, tumour necrosis factor (TNF)-? and granulocyte-macrophage colony-stimulating factor (GM-CSF) for at least 4 days post-transfection. Our results demonstrate that caTLR-4 is capable of exerting multiple T cell-enhancing effects and can potentially be used as a genetic adjuvant in adoptive cell therapy. Twit Text FOAVip Messenger RNA encoding constitutively active Toll-like receptor 4 enhances effector functions of human T cells ????Clin Exp Immunol http://www.kidney.de/pget.php?&tw=1&pmid=26212048 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26212048 #FOAvip English Wikipedia <ref>{{Cite pmid|26212048 }}</ref> Messenger RNA encoding constitutively active Toll-like receptor 4 enhances effector functions of human T cells #MMPMID26212048 Pato A ; Eisenberg G ; Machlenkin A ; Margalit A ; Cafri G ; Frankenburg S ; Merims S ; Peretz T ; Lotem M ; Gross G Clin Exp Immunol 2015[Nov]; 182 (2 ): 220-9 PMID26212048 show ga Adoptive T cell therapy of cancer employs a large number of ex-vivo-propagated T cells which recognize their targets either by virtue of their endogenous T cell receptor (TCR) or via genetic reprogramming. However, both cell-extrinsic and intrinsic mechanisms often diminish the in-vivo potency of these therapeutic T cells, limiting their clinical efficacy and broader use. Direct activation of human T cells by Toll-like receptor (TLR) ligands induces T cell survival and proliferation, boosts the production of proinflammatory cytokines and augments resistance to regulatory T cell (Treg) suppression. Removal of the TLR ligand-binding region results in constitutive signalling triggered by the remaining cytosolic Toll/interleukin-1 receptor (TIR) domain. The use of such TIR domains therefore offers an ideal means for equipping anti-tumour T cells with the arsenal of functional attributes required for improving current clinical protocols. Here we show that constitutively active (ca)TLR-4 can be expressed efficiently in human T cells using mRNA electroporation. The mere expression of caTLR-4 mRNA in polyclonal CD8 and CD4 T cells induced the production of interferon (IFN)-?, triggered the surface expression of CD25, CD69 and 4-1BB and up-regulated a panel of cytokines and chemokines. In tumour-infiltrating lymphocytes prepared from melanoma patients, caTLR-4 induced robust IFN-? secretion in all samples tested. Furthermore, caTLR-4 enhanced the anti-melanoma cytolytic activity of tumour-infiltrating lymphocytes and augmented the secretion of IFN-?, tumour necrosis factor (TNF)-? and granulocyte-macrophage colony-stimulating factor (GM-CSF) for at least 4 days post-transfection. Our results demonstrate that caTLR-4 is capable of exerting multiple T cell-enhancing effects and can potentially be used as a genetic adjuvant in adoptive cell therapy. |Antigens, CD/immunology/metabolism [MESH] |Antigens, Differentiation, T-Lymphocyte/immunology/metabolism [MESH] |CD4-Positive T-Lymphocytes/*immunology/metabolism [MESH] |CD8-Positive T-Lymphocytes/*immunology/metabolism [MESH] |Cell Line, Tumor [MESH] |Cells, Cultured [MESH] |Chemokines/immunology/metabolism [MESH] |Cytokines/immunology/metabolism [MESH] |Electroporation [MESH] |Flow Cytometry [MESH] |Gene Expression/immunology [MESH] |Granulocyte-Macrophage Colony-Stimulating Factor/immunology/metabolism [MESH] |Humans [MESH] |Interferon-gamma/immunology/metabolism [MESH] |Interleukin-2 Receptor alpha Subunit/immunology/metabolism [MESH] |K562 Cells [MESH] |Lectins, C-Type/immunology/metabolism [MESH] |Lymphocytes, Tumor-Infiltrating/immunology/metabolism [MESH] |RNA, Messenger/genetics/*immunology [MESH] |Reverse Transcriptase Polymerase Chain Reaction [MESH] |Toll-Like Receptor 4/genetics/*immunology/metabolism [MESH] |Transfection/methods [MESH] |Tumor Necrosis Factor Receptor Superfamily, Member 9/immunology/metabolism [MESH]Messenger RNA encoding constitutively active Toll-like receptor 4 enha(epmc).pdf DeepDyve Pubget Overpricing