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10.2217/epi.15.33 http://scihub22266oqcxt.onion/10.2217/epi.15.33 C4607651!4607651!25881900     free     free     free       Epigenomics 2015 ; 7 (5): 695-706 Nephropedia Template TP {{tp|p=25881900|t=2015. MIRA-seq for DNA methylation analysis of CpG islands |pdf=|usr=}}{{25881900}} gab.com Text MIRA-seq for DNA methylation analysis of CpG islands JO: Epigenomics http://www.kidney.de/pget.php?&tw=1&pmid=25881900 #FOAvip Aim:: To develop a reliable method for whole genome analysis of DNA methylation. Materials & methods:: Genome-scale analysis of DNA methylation includes affinity-based approaches such as enrichment using methyl-CpG-binding proteins. One of these methods, the methylated-CpG island recovery assay (MIRA), is based on the high affinity of the MBD2b-MBD3L1 complex for CpG-methylated DNA. Here we provide a detailed description of MIRA and combine it with next generation sequencing platforms (MIRA-seq). Results:: We assessed the performance of MIRA-seq and compared the data with whole genome bisulfite sequencing. Conclusion:: MIRA-seq is a reliable, genome-scale DNA methylation analysis platform for scoring DNA methylation differences at CpG-rich genomic regions. The method is not limited by primer or probe design and is cost effective. Twit Text FOAVip MIRA-seq for DNA methylation analysis of CpG islands ????Epigenomics http://www.kidney.de/pget.php?&tw=1&pmid=25881900 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25881900 #FOAvip English Wikipedia <ref>{{Cite pmid|25881900}}</ref> MIRA-seq for DNA methylation analysis of CpG islands #MMPMID25881900 Jung M; Kadam S; Xiong W; Rauch TA; Jin SG; Pfeifer GP Epigenomics 2015[Aug]; 7 (5): 695-706 PMID25881900show ga Aim:: To develop a reliable method for whole genome analysis of DNA methylation. Materials & methods:: Genome-scale analysis of DNA methylation includes affinity-based approaches such as enrichment using methyl-CpG-binding proteins. One of these methods, the methylated-CpG island recovery assay (MIRA), is based on the high affinity of the MBD2b-MBD3L1 complex for CpG-methylated DNA. Here we provide a detailed description of MIRA and combine it with next generation sequencing platforms (MIRA-seq). Results:: We assessed the performance of MIRA-seq and compared the data with whole genome bisulfite sequencing. Conclusion:: MIRA-seq is a reliable, genome-scale DNA methylation analysis platform for scoring DNA methylation differences at CpG-rich genomic regions. The method is not limited by primer or probe design and is cost effective. äMIRA-seq for DNA methylation analysis of CpG islands (epmc).pdf DeepDyve Pubget Overpricing