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10.1038/srep15287 http://scihub22266oqcxt.onion/10.1038/srep15287 C4606813!4606813 !26469694     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26469694 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2015 ; 5 (ä): 15287 Nephropedia Template TP {{tp|p=26469694 |t=2015. Identification of novel sRNAs involved in biofilm formation, motility, and fimbriae formation in Escherichia coli |pdf=|usr=}}{{26469694 }} gab.com Text Identification of novel sRNAs involved in biofilm formation, motility, and fimbriae formation in Escherichia coli JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26469694 #FOAvip Bacterial small RNAs (sRNAs) are known regulators in many physiological processes. In Escherichia coli, a large number of sRNAs have been predicted, among which only about a hundred are experimentally validated. Despite considerable research, the majority of their functions remain uncovered. Therefore, collective analysis of the roles of sRNAs in specific cellular processes may provide an effective approach to identify their functions. Here, we constructed a collection of plasmids overexpressing 99 individual sRNAs, and analyzed their effects on biofilm formation and related phenotypes. Thirty-three sRNAs significantly affecting these cellular processes were identified. No consistent correlations were observed, except that all five sRNAs suppressing type I fimbriae inhibited biofilm formation. Interestingly, IS118, yet to be characterized, suppressed all the processes. Our data not only reveal potentially critical functions of individual sRNAs in biofilm formation and other phenotypes but also highlight the unexpected complexity of sRNA-mediated metabolic pathways leading to these processes. Twit Text FOAVip Identification of novel sRNAs involved in biofilm formation, motility, and fimbriae formation in Escherichia coli ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26469694 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26469694 #FOAvip English Wikipedia <ref>{{Cite pmid|26469694 }}</ref> Identification of novel sRNAs involved in biofilm formation, motility, and fimbriae formation in Escherichia coli #MMPMID26469694 Bak G ; Lee J ; Suk S ; Kim D ; Young Lee J ; Kim KS ; Choi BS ; Lee Y Sci Rep 2015[Oct]; 5 (ä): 15287 PMID26469694 show ga Bacterial small RNAs (sRNAs) are known regulators in many physiological processes. In Escherichia coli, a large number of sRNAs have been predicted, among which only about a hundred are experimentally validated. Despite considerable research, the majority of their functions remain uncovered. Therefore, collective analysis of the roles of sRNAs in specific cellular processes may provide an effective approach to identify their functions. Here, we constructed a collection of plasmids overexpressing 99 individual sRNAs, and analyzed their effects on biofilm formation and related phenotypes. Thirty-three sRNAs significantly affecting these cellular processes were identified. No consistent correlations were observed, except that all five sRNAs suppressing type I fimbriae inhibited biofilm formation. Interestingly, IS118, yet to be characterized, suppressed all the processes. Our data not only reveal potentially critical functions of individual sRNAs in biofilm formation and other phenotypes but also highlight the unexpected complexity of sRNA-mediated metabolic pathways leading to these processes. |*Biofilms [MESH] |Blotting, Northern [MESH] |Escherichia coli Proteins/genetics/metabolism [MESH] |Escherichia coli/*genetics/physiology [MESH] |Fimbriae, Bacterial/*genetics [MESH] |Gene Expression Regulation, Bacterial [MESH] |Phenotype [MESH] |Plasmids/genetics/metabolism [MESH]Identification of novel sRNAs involved in biofilm formation, motility,(epmc).pdf DeepDyve Pubget Overpricing