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10.1155/2015/986436 http://scihub22266oqcxt.onion/10.1155/2015/986436 C4606214!4606214 !26495320     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26495320 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biomed+Res+Int 2015 ; 2015 (ä): 986436 Nephropedia Template TP {{tp|p=26495320 |t=2015. Shaped 3D singular spectrum analysis for quantifying gene expression, with application to the early zebrafish embryo |pdf=|usr=}}{{26495320 }} gab.com Text Shaped 3D singular spectrum analysis for quantifying gene expression, with application to the early zebrafish embryo JO: Biomed Res Int http://www.kidney.de/pget.php?&tw=1&pmid=26495320 #FOAvip Recent progress in microscopy technologies, biological markers, and automated processing methods is making possible the development of gene expression atlases at cellular-level resolution over whole embryos. Raw data on gene expression is usually very noisy. This noise comes from both experimental (technical/methodological) and true biological sources (from stochastic biochemical processes). In addition, the cells or nuclei being imaged are irregularly arranged in 3D space. This makes the processing, extraction, and study of expression signals and intrinsic biological noise a serious challenge for 3D data, requiring new computational approaches. Here, we present a new approach for studying gene expression in nuclei located in a thick layer around a spherical surface. The method includes depth equalization on the sphere, flattening, interpolation to a regular grid, pattern extraction by Shaped 3D singular spectrum analysis (SSA), and interpolation back to original nuclear positions. The approach is demonstrated on several examples of gene expression in the zebrafish egg (a model system in vertebrate development). The method is tested on several different data geometries (e.g., nuclear positions) and different forms of gene expression patterns. Fully 3D datasets for developmental gene expression are becoming increasingly available; we discuss the prospects of applying 3D-SSA to data processing and analysis in this growing field. Twit Text FOAVip Shaped 3D singular spectrum analysis for quantifying gene expression, with application to the early zebrafish embryo ????Biomed Res Int http://www.kidney.de/pget.php?&tw=1&pmid=26495320 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26495320 #FOAvip English Wikipedia <ref>{{Cite pmid|26495320 }}</ref> Shaped 3D singular spectrum analysis for quantifying gene expression, with application to the early zebrafish embryo #MMPMID26495320 Shlemov A ; Golyandina N ; Holloway D ; Spirov A Biomed Res Int 2015[]; 2015 (ä): 986436 PMID26495320 show ga Recent progress in microscopy technologies, biological markers, and automated processing methods is making possible the development of gene expression atlases at cellular-level resolution over whole embryos. Raw data on gene expression is usually very noisy. This noise comes from both experimental (technical/methodological) and true biological sources (from stochastic biochemical processes). In addition, the cells or nuclei being imaged are irregularly arranged in 3D space. This makes the processing, extraction, and study of expression signals and intrinsic biological noise a serious challenge for 3D data, requiring new computational approaches. Here, we present a new approach for studying gene expression in nuclei located in a thick layer around a spherical surface. The method includes depth equalization on the sphere, flattening, interpolation to a regular grid, pattern extraction by Shaped 3D singular spectrum analysis (SSA), and interpolation back to original nuclear positions. The approach is demonstrated on several examples of gene expression in the zebrafish egg (a model system in vertebrate development). The method is tested on several different data geometries (e.g., nuclear positions) and different forms of gene expression patterns. Fully 3D datasets for developmental gene expression are becoming increasingly available; we discuss the prospects of applying 3D-SSA to data processing and analysis in this growing field. |Animals [MESH] |Embryo, Mammalian/embryology/*metabolism [MESH] |Gene Expression Profiling/*methods [MESH] |Gene Expression Regulation, Developmental/*physiology [MESH] |Image Interpretation, Computer-Assisted/methods [MESH] |Imaging, Three-Dimensional/methods [MESH] |Microscopy, Fluorescence/*methods [MESH] |Reproducibility of Results [MESH] |Sensitivity and Specificity [MESH] |Spectrometry, Fluorescence/methods [MESH]Shaped 3D singular spectrum analysis for quantifying gene expression, (epmc).pdf DeepDyve Pubget Overpricing