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2012 ; 35
(5-6
): 407-20
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Multispectral enhancement method to increase the visual differences of tissue
structures in stained histopathology images
#MMPMID22935779
Bautista PA
; Yagi Y
Anal Cell Pathol (Amst)
2012[]; 35
(5-6
): 407-20
PMID22935779
show ga
In this paper we proposed a multispectral enhancement scheme in which the
spectral colors of the stained tissue-structure of interest and its background
can be independently modified by the user to further improve their visualization
and color discrimination. The colors of the background objects are modified by
transforming their N-band spectra through an NxN transformation matrix, which is
derived by mapping the representative samples of their original spectra to the
spectra of their target colors using least mean square method. On the other hand,
the color of the tissue structure of interest is modified by modulating the
transformed spectra with the sum of the pixel's spectral residual-errors at
specific bands weighted through an NxN weighting matrix; the spectral error is
derived by taking the difference between the pixel's original spectrum and its
reconstructed spectrum using the first M dominant principal component vectors in
principal component analysis. Promising results were obtained on the
visualization of the collagen fiber and the non-collagen tissue structures, e.g.,
nuclei, cytoplasm and red blood cells (RBC), in a hematoxylin and eosin (H&E)
stained image.