Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\23579249
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Anal+Cell+Pathol+(Amst)
2013 ; 36
(1-2
): 27-35
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Structured illumination-based super-resolution optical microscopy for hemato- and
cyto-pathology applications
#MMPMID23579249
Zhang T
; Osborn S
; Brandow C
; Dwyre D
; Green R
; Lane S
; Wachsmann-Hogiu S
Anal Cell Pathol (Amst)
2013[]; 36
(1-2
): 27-35
PMID23579249
show ga
Structured illumination fluorescence microscopy utilizes interfering light and
the moiré effect to enhance spatial resolution to about a half of that of
conventional light microscopy, i.e. approximately 90 nm. In addition to the
enhancement in the x and y directions, it also allows enhancement of resolution
in the z- direction by the same factor of two (to approximately 220 nm), making
it a powerful tool for 3-D morphology studies of fluorescently labeled cells or
thin tissue sections. In this report, we applied this technique to several types
of blood cells that are commonly seen in hematopathology. Compared with standard
brightfield and ordinary fluorescence microscopy images, the 3-D morphology
results clearly reveal the morphological features of different types of normal
blood cells. We have also used this technique to evaluate morphologies of
abnormal erythrocytes and compare them with those recorded on normal cells. The
results give a very intuitive presentation of morphological structures of
erythrocytes with great details. This research illustrates the potential of this
technique to be used in hematology and cyto-pathology studies aimed at
identifying nanometer-sized features that cannot be distinguished otherwise with
conventional optical microscopy.