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10.1089/adt.2015.669 http://scihub22266oqcxt.onion/10.1089/adt.2015.669 C4605357!4605357!26383544     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Assay+Drug+Dev+Technol 2015 ; 13 (8): 456-65 Nephropedia Template TP {{tp|p=26383544|t=2015. Bioluminescent, Nonlytic, Real-Time Cell Viability Assay and Use in Inhibitor Screening |pdf=|usr=}}{{26383544}} gab.com Text Bioluminescent, Nonlytic, Real-Time Cell Viability Assay and Use in Inhibitor Screening JO: Assay Drug Dev Technol http://www.kidney.de/pget.php?&tw=1&pmid=26383544 #FOAvip Real-time continuous monitoring of cellular processes offers distinct advantages over traditional endpoint assays. A comprehensive representation of the changes occurring in live cells over the entire length of an experiment provides information about the biological status of the cell and informs decisions about the timing of treatments or the use of other functional endpoint assays. We describe a homogeneous, nonlytic, bioluminescent assay that measures cell viability in real time. This time-dependent measurement allowed us to monitor cell health for 72?h from the same test samples, distinguish differential cell growth, and investigate drug mechanism of action by analyzing time- and dose-dependent drug effects. The real-time measurements also allowed us to detect cell death immediately (>75% signal decrease within 15?min of digitonin addition), analyze drug potency versus efficacy, and identify cytostatic versus toxic drug effects. We screened an oncology compound library (Z??=?0.7) and identified compounds with varying activity at different time points (1.6% of the library showed activity within 3?h, whereas 35.4% showed a response by 47?h). The assay compared well with orthogonal endpoint cell viability assays and additionally provided data at multiple time points and the opportunity to multiplex assays on the same cells. To test the advantage of time-dependent measurements to direct optimal timing of downstream applications, we used the real-time cell viability assay to determine the ideal time to measure caspase activity by monitoring the onset of cell death and multiplexing a luminescent caspase activation assay on the same test samples. Twit Text FOAVip Bioluminescent, Nonlytic, Real-Time Cell Viability Assay and Use in Inhibitor Screening ????Assay Drug Dev Technol http://www.kidney.de/pget.php?&tw=1&pmid=26383544 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26383544 #FOAvip English Wikipedia <ref>{{Cite pmid|26383544}}</ref> Bioluminescent, Nonlytic, Real-Time Cell Viability Assay and Use in Inhibitor Screening #MMPMID26383544 Duellman SJ; Zhou W; Meisenheimer P; Vidugiris G; Cali JJ; Gautam P; Wennerberg K; Vidugiriene J Assay Drug Dev Technol 2015[Oct]; 13 (8): 456-65 PMID26383544show ga Real-time continuous monitoring of cellular processes offers distinct advantages over traditional endpoint assays. A comprehensive representation of the changes occurring in live cells over the entire length of an experiment provides information about the biological status of the cell and informs decisions about the timing of treatments or the use of other functional endpoint assays. We describe a homogeneous, nonlytic, bioluminescent assay that measures cell viability in real time. This time-dependent measurement allowed us to monitor cell health for 72?h from the same test samples, distinguish differential cell growth, and investigate drug mechanism of action by analyzing time- and dose-dependent drug effects. The real-time measurements also allowed us to detect cell death immediately (>75% signal decrease within 15?min of digitonin addition), analyze drug potency versus efficacy, and identify cytostatic versus toxic drug effects. We screened an oncology compound library (Z??=?0.7) and identified compounds with varying activity at different time points (1.6% of the library showed activity within 3?h, whereas 35.4% showed a response by 47?h). The assay compared well with orthogonal endpoint cell viability assays and additionally provided data at multiple time points and the opportunity to multiplex assays on the same cells. To test the advantage of time-dependent measurements to direct optimal timing of downstream applications, we used the real-time cell viability assay to determine the ideal time to measure caspase activity by monitoring the onset of cell death and multiplexing a luminescent caspase activation assay on the same test samples. äBioluminescent, Nonlytic, Real-Time Cell Viability Assay and Use in In(epmc).pdf DeepDyve Pubget Overpricing