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10.1093/nar/gkv583 http://scihub22266oqcxt.onion/10.1093/nar/gkv583 C4605290!4605290 !26068470     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26068470 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nucleic+Acids+Res 2015 ; 43 (18 ): e120 Nephropedia Template TP {{tp|p=26068470 |t=2015. Efficient production of superior dumbbell-shaped DNA minimal vectors for small hairpin RNA expression |pdf=|usr=}}{{26068470 }} gab.com Text Efficient production of superior dumbbell-shaped DNA minimal vectors for small hairpin RNA expression JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=26068470 #FOAvip Genetic therapy holds great promise for the treatment of inherited or acquired genetic diseases; however, its breakthrough is hampered by the lack of suitable gene delivery systems. Dumbbell-shaped DNA minimal vectors represent an attractive, safe alternative to the commonly used viral vectors which are fraught with risk, but dumbbell generation appears to be costly and time-consuming. We developed a new PCR-based method for dumbbell production which comprises only two steps. First, PCR amplification of the therapeutic expression cassette using chemically modified primers to form a ready-to-ligate DNA structure; and second, a highly efficient intramolecular ligation reaction. Compared with conventional strategies, the new method produces dumbbell vectors more rapidly, with higher yields and purity, and at lower costs. In addition, such produced small hairpin RNA expressing dumbbells triggered superior target gene knockdown compared with conventionally produced dumbbells or plasmids. Our novel method is suitable for large-scale dumbbell production and can facilitate clinical applications of this vector system. Twit Text FOAVip Efficient production of superior dumbbell-shaped DNA minimal vectors for small hairpin RNA expression ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=26068470 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26068470 #FOAvip English Wikipedia <ref>{{Cite pmid|26068470 }}</ref> Efficient production of superior dumbbell-shaped DNA minimal vectors for small hairpin RNA expression #MMPMID26068470 Yu H ; Jiang X ; Tan KT ; Hang L ; Patzel V Nucleic Acids Res 2015[Oct]; 43 (18 ): e120 PMID26068470 show ga Genetic therapy holds great promise for the treatment of inherited or acquired genetic diseases; however, its breakthrough is hampered by the lack of suitable gene delivery systems. Dumbbell-shaped DNA minimal vectors represent an attractive, safe alternative to the commonly used viral vectors which are fraught with risk, but dumbbell generation appears to be costly and time-consuming. We developed a new PCR-based method for dumbbell production which comprises only two steps. First, PCR amplification of the therapeutic expression cassette using chemically modified primers to form a ready-to-ligate DNA structure; and second, a highly efficient intramolecular ligation reaction. Compared with conventional strategies, the new method produces dumbbell vectors more rapidly, with higher yields and purity, and at lower costs. In addition, such produced small hairpin RNA expressing dumbbells triggered superior target gene knockdown compared with conventionally produced dumbbells or plasmids. Our novel method is suitable for large-scale dumbbell production and can facilitate clinical applications of this vector system. |Cell Line [MESH] |DNA Primers [MESH] |DNA/chemistry [MESH] |Furans/chemistry [MESH] |Gene Expression [MESH] |Gene Knockout Techniques [MESH] |Genetic Vectors/*biosynthesis/*chemistry [MESH] |Humans [MESH] |Polymerase Chain Reaction/economics/*methods [MESH]Efficient production of superior dumbbell-shaped DNA minimal vectors f(epmc).pdf DeepDyve Pubget Overpricing