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10.1007/978-1-62703-637-5_10

http://scihub22266oqcxt.onion/10.1007/978-1-62703-637-5_10
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C4605221!4605221!24014405
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suck abstract from ncbi


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pmid24014405      Methods+Mol+Biol 2013 ; 1077 (ä): 149-63
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  • METHODS IN MOLECULAR BIOLOGY: ASSAYING CHROMATIN SIRTUINS #MMPMID24014405
  • Zhong L; Martinez-Pastor B; Silberman DM; Sebastian C; Mostoslavsky R
  • Methods Mol Biol 2013[]; 1077 (ä): 149-63 PMID24014405show ga
  • Most of the sirtuins? nuclear substrates indentified so far are histones or other chromatin-associated proteins and, thus, it is of special relevance the development of good biochemical techniques to analyze the biology of these proteins in the context of chromatin. Here, we describe several of the chromatin-based techniques to identify sirtuins? substrates, including a chromatin immunoprecipitation (ChIP) protocol, an acid-extraction protocol, and a nucleosomal immunoprecipitation protocol to analyze putative sirtuin chromatin interactors.
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