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2015 ; 6
(10
): 3783-94
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Red emissive AIE nanodots with high two-photon absorption efficiency at 1040 nm
for deep-tissue in vivo imaging
#MMPMID26504629
Wang Y
; Hu R
; Xi W
; Cai F
; Wang S
; Zhu Z
; Bai R
; Qian J
Biomed Opt Express
2015[Oct]; 6
(10
): 3783-94
PMID26504629
show ga
Deep-tissue penetration is highly required in in vivo optical bioimaging. We
synthesized a type of red emissive fluorophore BT with aggregation-induced
emission (AIE) property. BT molecules were then encapsulated with amphiphilic
polymers to form nanodots, and a large two-photon absorption (2PA) cross-section
of 2.9 × 10(6) GM at 1040 nm was observed from each BT nanodot, which was much
larger than those at the wavelengths of 770 to 860 nm. In addition, 1040 nm light
was found to have better penetration and focusing capability than 800 nm light in
biological tissue, according to the Monte Carlo simulation. The toxicity and
tissue distribution of BT nanodots were studied, and they were found to have good
biocompatibility. BT nanodots were then utilized for in vivo imaging of mouse ear
and brain, and an imaging depth of 700 ?m was obtained with the femtosecond (fs)
excitation of 1040 nm. The red emissive AIE nanodots with high 2PA efficiency at
1040 nm would be useful for deep-tissue functional bioimaging in the future.