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10.1364/BOE.6.003724 http://scihub22266oqcxt.onion/10.1364/BOE.6.003724 C4605033!4605033!26504624     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomed+Opt+Express 2015 ; 6 (10): 3724-36 Nephropedia Template TP {{tp|p=26504624|t=2015. Handheld imaging photonic crystal biosensor for multiplexed, label-free protein detection |pdf=|usr=}}{{26504624}} gab.com Text Handheld imaging photonic crystal biosensor for multiplexed, label-free protein detection JO: Biomed Opt Express http://www.kidney.de/pget.php?&tw=1&pmid=26504624 #FOAvip We present a handheld biosensor system for the label-free and specific multiplexed detection of several biomarkers employing a spectrometer-free imaging measurement system. A photonic crystal surface functionalized with multiple specific ligands forms the optical transducer. The photonic crystal slab is fabricated on a glass substrate by replicating a periodic grating master stamp with a period of 370 nm into a photoresist via nanoimprint lithography and deposition of a 70-nm titanium dioxide layer. Capture molecules are coupled covalently and drop-wise to the photonic crystal surface. With a simple camera and imaging optics the surface-normal transmission is detected. In the transmission spectrum guided-mode resonances are observed that shift due to protein binding. This shift is observed as an intensity change in the green color channel of the camera. Non-functionalized image sections are used for continuous elimination of background drift. In a first experiment we demonstrate the specific and time-resolved detection of 90.0 nm CD40 ligand antibody, 90.0 nM EGF antibody, and 500 nM streptavidin in parallel on one sensor chip. In a second experiment, aptamers with two different spacer lengths are used as receptor. The binding kinetics with association and dissociation of 250 nM thrombin and regeneration of the sensor surface with acidic tris-HCl-buffer (pH 5.0) is presented for two measurement cycles. Twit Text FOAVip Handheld imaging photonic crystal biosensor for multiplexed, label-free protein detection ????Biomed Opt Express http://www.kidney.de/pget.php?&tw=1&pmid=26504624 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26504624 #FOAvip English Wikipedia <ref>{{Cite pmid|26504624}}</ref> Handheld imaging photonic crystal biosensor for multiplexed, label-free protein detection #MMPMID26504624 Jahns S; Bräu M; Meyer BO; Karrock T; Gutekunst SB; Blohm L; Selhuber-Unkel C; Buhmann R; Nazirizadeh Y; Gerken M Biomed Opt Express 2015[Oct]; 6 (10): 3724-36 PMID26504624show ga We present a handheld biosensor system for the label-free and specific multiplexed detection of several biomarkers employing a spectrometer-free imaging measurement system. A photonic crystal surface functionalized with multiple specific ligands forms the optical transducer. The photonic crystal slab is fabricated on a glass substrate by replicating a periodic grating master stamp with a period of 370 nm into a photoresist via nanoimprint lithography and deposition of a 70-nm titanium dioxide layer. Capture molecules are coupled covalently and drop-wise to the photonic crystal surface. With a simple camera and imaging optics the surface-normal transmission is detected. In the transmission spectrum guided-mode resonances are observed that shift due to protein binding. This shift is observed as an intensity change in the green color channel of the camera. Non-functionalized image sections are used for continuous elimination of background drift. In a first experiment we demonstrate the specific and time-resolved detection of 90.0 nm CD40 ligand antibody, 90.0 nM EGF antibody, and 500 nM streptavidin in parallel on one sensor chip. In a second experiment, aptamers with two different spacer lengths are used as receptor. The binding kinetics with association and dissociation of 250 nM thrombin and regeneration of the sensor surface with acidic tris-HCl-buffer (pH 5.0) is presented for two measurement cycles. äHandheld imaging photonic crystal biosensor for multiplexed, label-fre(epmc).pdf DeepDyve Pubget Overpricing