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10.1016/j.jss.2015.04.076 http://scihub22266oqcxt.onion/10.1016/j.jss.2015.04.076 C4604002!4604002!25990696     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Surg+Res 2015 ; 199 (1): 1-6 Nephropedia Template TP {{tp|p=25990696|t=2015. Matrix Gla Protein Regulates Calcification of the Aortic Valve |pdf=|usr=}}{{25990696}} gab.com Text Matrix Gla Protein Regulates Calcification of the Aortic Valve JO: J Surg Res http://www.kidney.de/pget.php?&tw=1&pmid=25990696 #FOAvip BACKGROUND: The aortic valve interstitial cell (AVIC) has been implicated in the pathogenesis of aortic stenosis. In response to pro-inflammatory stimulation, the AVIC undergoes a phenotypic change from that of a myofibroblast phenotype to that of osteoblast-like cell. Matrix gla-protein (MGP) has been identified as an important inhibitor of vascular calcification. We therefore hypothesized that MGP expression is reduced in diseased AVICs, and loss of this protective protein contributes to calcification of the aortic valve. Our purpose was to compare MGP expression in normal vs. diseased AVICs. MATERIALS AND METHODS: Human AVICs were isolated from normal aortic valves from explanted hearts (n=6) at the time of heart transplantation. AVICs were also isolated from calcified, diseased valves of patients (n=6) undergoing aortic valve replacement. AVICs were grown in culture until they reached passages 2?6 prior to experimentation. Immunofluorescent staining (IF), RT-PCR, immunoblotting (IB), and ELISA were used to compare levels of MGP in normal and diseased AVICs. Statistics were by Mann Whitney U test (p<0.05). RESULTS: MGP expression was significantly decreased in diseased AVICs relative to normal AVICs by IF, RT-PCR, IB, and ELISA. CONCLUSIONS: An important anti-calcification defense mechanism is deficient in calcified aortic valves. MGP expression is significantly lower in diseased relative to normal AVICs. Lack of this important ?anti-calcification? protein may contribute to calcification of the aortic valve. Twit Text FOAVip Matrix Gla Protein Regulates Calcification of the Aortic Valve ????J Surg Res http://www.kidney.de/pget.php?&tw=1&pmid=25990696 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25990696 #FOAvip English Wikipedia <ref>{{Cite pmid|25990696}}</ref> Matrix Gla Protein Regulates Calcification of the Aortic Valve #MMPMID25990696 Venardos N; Bennett D; Weyant MJ; Reece TB; Meng X; Fullerton DA J Surg Res 2015[Nov]; 199 (1): 1-6 PMID25990696show ga BACKGROUND: The aortic valve interstitial cell (AVIC) has been implicated in the pathogenesis of aortic stenosis. In response to pro-inflammatory stimulation, the AVIC undergoes a phenotypic change from that of a myofibroblast phenotype to that of osteoblast-like cell. Matrix gla-protein (MGP) has been identified as an important inhibitor of vascular calcification. We therefore hypothesized that MGP expression is reduced in diseased AVICs, and loss of this protective protein contributes to calcification of the aortic valve. Our purpose was to compare MGP expression in normal vs. diseased AVICs. MATERIALS AND METHODS: Human AVICs were isolated from normal aortic valves from explanted hearts (n=6) at the time of heart transplantation. AVICs were also isolated from calcified, diseased valves of patients (n=6) undergoing aortic valve replacement. AVICs were grown in culture until they reached passages 2?6 prior to experimentation. Immunofluorescent staining (IF), RT-PCR, immunoblotting (IB), and ELISA were used to compare levels of MGP in normal and diseased AVICs. Statistics were by Mann Whitney U test (p<0.05). RESULTS: MGP expression was significantly decreased in diseased AVICs relative to normal AVICs by IF, RT-PCR, IB, and ELISA. CONCLUSIONS: An important anti-calcification defense mechanism is deficient in calcified aortic valves. MGP expression is significantly lower in diseased relative to normal AVICs. Lack of this important ?anti-calcification? protein may contribute to calcification of the aortic valve. äMatrix Gla Protein Regulates Calcification of the Aortic Valve (epmc).pdf DeepDyve Pubget Overpricing