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10.1007/s00335-015-9603-x http://scihub22266oqcxt.onion/10.1007/s00335-015-9603-x C4602060!4602060 !26449678     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26449678 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mamm+Genome 2015 ; 26 (9-10 ): 486-500 Nephropedia Template TP {{tp|p=26449678 |t=2015. Current strategies for mutation detection in phenotype-driven screens utilising next generation sequencing |pdf=|usr=}}{{26449678 }} gab.com Text Current strategies for mutation detection in phenotype-driven screens utilising next generation sequencing JO: Mamm Genome http://www.kidney.de/pget.php?&tw=1&pmid=26449678 #FOAvip Mutagenesis-based screens in mice are a powerful discovery platform to identify novel genes or gene functions associated with disease phenotypes. An N-ethyl-N-nitrosourea (ENU) mutagenesis screen induces single nucleotide variants randomly in the mouse genome. Subsequent phenotyping of mutant and wildtype mice enables the identification of mutated pathways resulting in phenotypes associated with a particular ENU lesion. This unbiased approach to gene discovery conducts the phenotyping with no prior knowledge of the functional mutations. Before the advent of affordable next generation sequencing (NGS), ENU variant identification was a limiting step in gene characterization, akin to 'finding a needle in a haystack'. The emergence of a reliable reference genome alongside advances in NGS has propelled ENU mutation discovery from an arduous, time-consuming exercise to an effective and rapid form of mutation discovery. This has permitted large mouse facilities worldwide to use ENU for novel mutation discovery in a high-throughput manner, helping to accelerate basic science at the mechanistic level. Here, we describe three different strategies used to identify ENU variants from NGS data and some of the subsequent steps for mutation characterisation. Twit Text FOAVip Current strategies for mutation detection in phenotype-driven screens utilising next generation sequencing ????Mamm Genome http://www.kidney.de/pget.php?&tw=1&pmid=26449678 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26449678 #FOAvip English Wikipedia <ref>{{Cite pmid|26449678 }}</ref> Current strategies for mutation detection in phenotype-driven screens utilising next generation sequencing #MMPMID26449678 Simon MM ; Moresco EM ; Bull KR ; Kumar S ; Mallon AM ; Beutler B ; Potter PK Mamm Genome 2015[Oct]; 26 (9-10 ): 486-500 PMID26449678 show ga Mutagenesis-based screens in mice are a powerful discovery platform to identify novel genes or gene functions associated with disease phenotypes. An N-ethyl-N-nitrosourea (ENU) mutagenesis screen induces single nucleotide variants randomly in the mouse genome. Subsequent phenotyping of mutant and wildtype mice enables the identification of mutated pathways resulting in phenotypes associated with a particular ENU lesion. This unbiased approach to gene discovery conducts the phenotyping with no prior knowledge of the functional mutations. Before the advent of affordable next generation sequencing (NGS), ENU variant identification was a limiting step in gene characterization, akin to 'finding a needle in a haystack'. The emergence of a reliable reference genome alongside advances in NGS has propelled ENU mutation discovery from an arduous, time-consuming exercise to an effective and rapid form of mutation discovery. This has permitted large mouse facilities worldwide to use ENU for novel mutation discovery in a high-throughput manner, helping to accelerate basic science at the mechanistic level. Here, we describe three different strategies used to identify ENU variants from NGS data and some of the subsequent steps for mutation characterisation. |*High-Throughput Nucleotide Sequencing [MESH] |Animals [MESH] |Disease Models, Animal [MESH] |Ethylnitrosourea/toxicity [MESH] |Genome/*drug effects [MESH] |Mice [MESH] |Mutagenesis/drug effects/genetics [MESH]Current strategies for mutation detection in phenotype-driven screens (epmc).pdf DeepDyve Pubget Overpricing