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2015 ; 10
(10
): e0138276
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English Wikipedia
A SWI/SNF Chromatin Remodelling Protein Controls Cytokinin Production through the
Regulation of Chromatin Architecture
#MMPMID26457678
Jégu T
; Domenichini S
; Blein T
; Ariel F
; Christ A
; Kim SK
; Crespi M
; Boutet-Mercey S
; Mouille G
; Bourge M
; Hirt H
; Bergounioux C
; Raynaud C
; Benhamed M
PLoS One
2015[]; 10
(10
): e0138276
PMID26457678
show ga
Chromatin architecture determines transcriptional accessibility to DNA and
consequently gene expression levels in response to developmental and
environmental stimuli. Recently, chromatin remodelers such as SWI/SNF complexes
have been recognized as key regulators of chromatin architecture. To gain insight
into the function of these complexes during root development, we have analyzed
Arabidopsis knock-down lines for one sub-unit of SWI/SNF complexes: BAF60. Here,
we show that BAF60 is a positive regulator of root development and cell cycle
progression in the root meristem via its ability to down-regulate cytokinin
production. By opposing both the deposition of active histone marks and the
formation of a chromatin regulatory loop, BAF60 negatively regulates two crucial
target genes for cytokinin biosynthesis (IPT3 and IPT7) and one cell cycle
inhibitor (KRP7). Our results demonstrate that SWI/SNF complexes containing BAF60
are key factors governing the equilibrium between formation and dissociation of a
chromatin loop controlling phytohormone production and cell cycle progression.