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10.1002/anie.201504287 http://scihub22266oqcxt.onion/10.1002/anie.201504287 C4600229!4600229!26331292     free     free     free       Angew+Chem+Int+Ed+Engl 2015 ; 54 (41): 11952-5 Nephropedia Template TP {{tp|p=26331292|t=2015. An Accurate In Vitro Model of the E coli Envelope |pdf=|usr=}}{{26331292}} gab.com Text An Accurate In Vitro Model of the E coli Envelope JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=26331292 #FOAvip Gram-negative bacteria are an increasingly serious source of antibiotic-resistant infections, partly owing to their characteristic protective envelope. This complex, 20 nm thick barrier includes a highly impermeable, asymmetric bilayer outer membrane (OM), which plays a pivotal role in resisting antibacterial chemotherapy. Nevertheless, the OM molecular structure and its dynamics are poorly understood because the structure is difficult to recreate or study in vitro. The successful formation and characterization of a fully asymmetric model envelope using Langmuir?Blodgett and Langmuir?Schaefer methods is now reported. Neutron reflectivity and isotopic labeling confirmed the expected structure and asymmetry and showed that experiments with antibacterial proteins reproduced published in vivo behavior. By closely recreating natural OM behavior, this model provides a much needed robust system for antibiotic development. Twit Text FOAVip An Accurate In Vitro Model of the E coli Envelope ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=26331292 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26331292 #FOAvip English Wikipedia <ref>{{Cite pmid|26331292}}</ref> An Accurate In Vitro Model of the E coli Envelope #MMPMID26331292 Clifton LA; Holt SA; Hughes AV; Daulton EL; Arunmanee W; Heinrich F; Khalid S; Jefferies D; Charlton TR; Webster JRP; Kinane CJ; Lakey JH Angew Chem Int Ed Engl 2015[Oct]; 54 (41): 11952-5 PMID26331292show ga Gram-negative bacteria are an increasingly serious source of antibiotic-resistant infections, partly owing to their characteristic protective envelope. This complex, 20 nm thick barrier includes a highly impermeable, asymmetric bilayer outer membrane (OM), which plays a pivotal role in resisting antibacterial chemotherapy. Nevertheless, the OM molecular structure and its dynamics are poorly understood because the structure is difficult to recreate or study in vitro. The successful formation and characterization of a fully asymmetric model envelope using Langmuir?Blodgett and Langmuir?Schaefer methods is now reported. Neutron reflectivity and isotopic labeling confirmed the expected structure and asymmetry and showed that experiments with antibacterial proteins reproduced published in vivo behavior. By closely recreating natural OM behavior, this model provides a much needed robust system for antibiotic development. äAn Accurate In Vitro Model of the E coli Envelope (epmc).pdf DeepDyve Pubget Overpricing