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2015 ; 226
(10
): 353
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Biological Activity Assessment in Mexican Tropical Soils with Different
Hydrocarbon Contamination Histories
#MMPMID26478633
Riveroll-Larios J
; Escalante-Espinosa E
; Fócil-Monterrubio RL
; Díaz-Ramírez IJ
Water Air Soil Pollut
2015[]; 226
(10
): 353
PMID26478633
show ga
The use of soil health indicators linked to microbial activities, such as key
enzymes and respirometric profiles, helps assess the natural attenuation
potential of soils contaminated with hydrocarbons. In this study, the intrinsic
physicochemical characteristics, biological activity and biodegradation potential
were recorded for two soils with different contamination histories (>5 years and
<1 months). The enzymatic activity (lipase and dehydrogenase) as well as
microbiological and mineralisation profiles were measured in contaminated soil
samples. Soil suspensions were tested as microbial inocula in biodegradation
potential assays using contaminated perlite as an inert support. The basal
respiratory rate of the recently contaminated soil was 15-38 mg C-CO(2)?kg(-1)
h(-1), while the weathered soil presented a greater basal mineralisation capacity
of 55-70 mg C-CO(2) kg(-1) h(-1). The basal levels of lipase and dehydrogenase
were significantly greater than those recorded in non-contaminated soils
(551?±?21 ?g pNP g(-1)). Regarding the biodegradation potential assessment, the
lipase (1000-3000 ?g pNP g(-1) of perlite) and dehydrogenase (~3000 ?g INF g(-1)
of perlite) activities in the inoculum of the recently contaminated soil were
greater than those recorded in the inoculum of the weathered soil. This was
correlated with a high mineralisation rate (~30 mg C-CO(2) kg(-1) h(-1)) in the
recently contaminated soil and a reduction in hydrocarbon concentration (~30 %).
The combination of an inert support and enzymatic and respirometric analyses made
it possible to detect the different biodegradation capacities of the studied
inocula and the natural attenuation potential of a recently contaminated soil at
high hydrocarbon concentrations.