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2015 ; 10
(ä): 23-7
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Organophosphate Hydrolase in Conductometric Biosensor for the Detection of
Organophosphate Pesticides
#MMPMID26483607
Mulyasuryani A
; Prasetyawan S
Anal Chem Insights
2015[]; 10
(ä): 23-7
PMID26483607
show ga
The research has developed an enzyme biosensor for the detection organophosphate
pesticide residues. The biosensor consists of a pair of screen-printed carbon
electrode (SPCEs). One of electrodes contains immobilized organophosphate
hydrolase (OPH) on a chitosan membrane by cross-linking it with glutaraldehyde.
The area of the electrodes was optimized to 3, 5, and 7 mm(2). The OPH was
isolated from Pseudomonas putida, and was purified by the ammonium sulfate
precipitation method, with 6444 ppm (A) and 7865 ppm (B). The organophosphate
pesticide samples were 0-100 ppb in tris-acetate buffer 0.05 M, pH 8.5. The
results showed that the best performance of the biosensor was achieved by the
enzyme A with an electrode area of 5 mm(2). The sensitivity of the biosensor was
between 3 and 32 µS/ppb, and the detection limit for the organophosphate
pesticides was 40 ppb (diazinon), 30 ppb (malathion), 20 ppb (chlorpyrifos), and
40 ppm (profenofos).