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2015 ; 59
(3
): 2539
Nephropedia Template TP
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Three-dimensional apoptotic nuclear behavior analyzed by means of Field Emission
in Lens Scanning Electron Microscope
#MMPMID26428889
Salucci S
; Burattini S
; Falcieri E
; Gobbi P
Eur J Histochem
2015[Sep]; 59
(3
): 2539
PMID26428889
show ga
Apoptosis is an essential biological function required during embryogenesis,
tissue homeostasis, organ development and immune system regulation. It is an
active cell death pathway involved in a variety of pathological conditions.
During this process cytoskeletal proteins appear damaged and undergo an enzymatic
disassembling, leading to formation of apoptotic features. This study was
designed to examine the three-dimensional chromatin behavior and cytoskeleton
involvement, in particular actin re-modeling. HL-60 cells, exposed to
hyperthermia, a known apoptotic trigger, were examined by means of a Field
Emission in Lens Scanning Electron Microscope (FEISEM). Ultrastructural
observations revealed in treated cells the presence of apoptotic patterns after
hyperthermia trigger. In particular, three-dimensional apoptotic chromatin
rearrangements appeared involving the translocation of filamentous actin from
cytoplasm to the nucleus. FEISEM immunogold techniques showed actin labeling and
its precise three-dimensional localization in the diffuse chromatin, well
separated from the condensed one. The actin presence in dispersed chromatin
inside the apoptotic nucleus can be considered an important feature,
indispensable to permit the apoptotic machinery evolution.