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2015 ; 5
(ä): 14982
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Ultra-high-resolution 3D digitalized imaging of the cerebral angioarchitecture in
rats using synchrotron radiation
#MMPMID26443231
Zhang MQ
; Zhou L
; Deng QF
; Xie YY
; Xiao TQ
; Cao YZ
; Zhang JW
; Chen XM
; Yin XZ
; Xiao B
Sci Rep
2015[Oct]; 5
(ä): 14982
PMID26443231
show ga
The angioarchitecture is a fundamental aspect of brain development and
physiology. However, available imaging tools are unsuited for non-destructive
cerebral mapping of the functionally important three-dimensional (3D) vascular
microstructures. To address this issue, we developed an ultra-high resolution 3D
digitalized angioarchitectural map for rat brain, based on synchrotron radiation
phase contrast imaging (SR-PCI) with pixel size of 5.92??m. This approach
provides a systematic and detailed view of the cerebrovascular anatomy at the
micrometer level without any need for contrast agents. From qualitative and
quantitative perspectives, the present 3D data provide a considerable insight
into the spatial vascular network for whole rodent brain, particularly for
functionally important regions of interest, such as the hippocampus, pre-frontal
cerebral cortex and the corpus striatum. We extended these results to
synchrotron-based virtual micro-endoscopy, thus revealing the trajectory of
targeted vessels in 3D. The SR-PCI method for systematic visualization of
cerebral microvasculature holds considerable promise for wider application in
life sciences, including 3D micro-imaging in experimental models of
neurodevelopmental and vascular disorders.