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2015 ; 5
(ä): 14735
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Ultra-high voltage electron microscopy of primitive algae illuminates 3D
ultrastructures of the first photosynthetic eukaryote
#MMPMID26439276
Takahashi T
; Nishida T
; Saito C
; Yasuda H
; Nozaki H
Sci Rep
2015[Oct]; 5
(ä): 14735
PMID26439276
show ga
A heterotrophic organism 1-2 billion years ago enslaved a cyanobacterium to
become the first photosynthetic eukaryote, and has diverged globally. The primary
phototrophs, glaucophytes, are thought to retain ancestral features of the first
photosynthetic eukaryote, but examining the protoplast ultrastructure has
previously been problematic in the coccoid glaucophyte Glaucocystis due to its
thick cell wall. Here, we examined the three-dimensional (3D) ultrastructure in
two divergent species of Glaucocystis using ultra-high voltage electron
microscopy. Three-dimensional modelling of Glaucocystis cells using electron
tomography clearly showed that numerous, leaflet-like flattened vesicles are
distributed throughout the protoplast periphery just underneath a single-layered
plasma membrane. This 3D feature is essentially identical to that of another
glaucophyte genus Cyanophora, as well as the secondary phototrophs in Alveolata.
Thus, the common ancestor of glaucophytes and/or the first photosynthetic
eukaryote may have shown similar 3D structures.