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10.4161/isl.28095 http://scihub22266oqcxt.onion/10.4161/isl.28095 C4593566!4593566 !25483880     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25483880 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Islets 2014 ; 6 (1 ): e28095 Nephropedia Template TP {{tp|p=25483880 |t=2014. Optogenetic control of insulin secretion in intact pancreatic islets with ?-cell-specific expression of Channelrhodopsin-2 |pdf=|usr=}}{{25483880 }} gab.com Text Optogenetic control of insulin secretion in intact pancreatic islets with -cell-specific expression of Channelrhodopsin-2 JO: Islets http://www.kidney.de/pget.php?&tw=1&pmid=25483880 #FOAvip Insulin is secreted from the pancreatic ?-cells in response to elevated glucose. In intact islets the capacity for insulin release is determined by a complex interplay between different cell types. This has made it difficult to specifically assess the role of ?-cell defects to the insulin secretory impairment in type 2 diabetes. Here we describe a new approach, based on optogenetics, that enables specific investigation of ?-cells in intact islets. We used transgenic mice expressing the light-sensitive cation channel Channelrhodopsin-2 (ChR2) under control of the insulin promoter. Glucose tolerance in vivo was assessed using intraperitoneal glucose tolerance tests, and glucose-induced insulin release was measured from static batch incubations. ChR2 localization was determined by fluorescence confocal microscopy. The effect of ChR2 stimulation with blue LED light was assessed using Ca(2+) imaging and static islet incubations. Light stimulation of islets from transgenic ChR2 mice triggered prompt increases in intracellular Ca(2+). Moreover, light stimulation enhanced insulin secretion in batch-incubated islets at low and intermediate but not at high glucose concentrations. Glucagon release was not affected. Beta-cells from mice rendered diabetic on a high-fat diet exhibited a 3.5-fold increase in light-induced Ca(2+) influx compared with mice on a control diet. Furthermore, light enhanced insulin release also at high glucose in these mice, suggesting that high-fat feeding leads to a compensatory potentiation of the Ca(2+) response in ?-cells. The results demonstrate the usefulness and versatility of optogenetics for studying mechanisms of perturbed hormone secretion in diabetes with high time-resolution and cell-specificity. Twit Text FOAVip Optogenetic control of insulin secretion in intact pancreatic islets with -cell-specific expression of Channelrhodopsin-2 ????Islets http://www.kidney.de/pget.php?&tw=1&pmid=25483880 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25483880 #FOAvip English Wikipedia <ref>{{Cite pmid|25483880 }}</ref> Optogenetic control of insulin secretion in intact pancreatic islets with ?-cell-specific expression of Channelrhodopsin-2 #MMPMID25483880 Reinbothe TM ; Safi F ; Axelsson AS ; Mollet IG ; Rosengren AH Islets 2014[]; 6 (1 ): e28095 PMID25483880 show ga Insulin is secreted from the pancreatic ?-cells in response to elevated glucose. In intact islets the capacity for insulin release is determined by a complex interplay between different cell types. This has made it difficult to specifically assess the role of ?-cell defects to the insulin secretory impairment in type 2 diabetes. Here we describe a new approach, based on optogenetics, that enables specific investigation of ?-cells in intact islets. We used transgenic mice expressing the light-sensitive cation channel Channelrhodopsin-2 (ChR2) under control of the insulin promoter. Glucose tolerance in vivo was assessed using intraperitoneal glucose tolerance tests, and glucose-induced insulin release was measured from static batch incubations. ChR2 localization was determined by fluorescence confocal microscopy. The effect of ChR2 stimulation with blue LED light was assessed using Ca(2+) imaging and static islet incubations. Light stimulation of islets from transgenic ChR2 mice triggered prompt increases in intracellular Ca(2+). Moreover, light stimulation enhanced insulin secretion in batch-incubated islets at low and intermediate but not at high glucose concentrations. Glucagon release was not affected. Beta-cells from mice rendered diabetic on a high-fat diet exhibited a 3.5-fold increase in light-induced Ca(2+) influx compared with mice on a control diet. Furthermore, light enhanced insulin release also at high glucose in these mice, suggesting that high-fat feeding leads to a compensatory potentiation of the Ca(2+) response in ?-cells. The results demonstrate the usefulness and versatility of optogenetics for studying mechanisms of perturbed hormone secretion in diabetes with high time-resolution and cell-specificity. |*Optogenetics [MESH] |Animals [MESH] |Bacterial Proteins/genetics/metabolism [MESH] |Cell Tracking/methods [MESH] |Cells, Cultured [MESH] |Channelrhodopsins [MESH] |Insulin Secretion [MESH] |Insulin-Secreting Cells/*metabolism [MESH] |Insulin/genetics/*metabolism [MESH] |Luminescent Proteins/genetics/metabolism [MESH] |Mice [MESH] |Mice, Transgenic [MESH] |Organ Specificity/genetics [MESH] |Promoter Regions, Genetic [MESH]Optogenetic control of insulin secretion in intact pancreatic islets w(epmc).pdf DeepDyve Pubget Overpricing