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10.1002/anie.201403349 http://scihub22266oqcxt.onion/10.1002/anie.201403349 C4593319!4593319!25081303     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Angew+Chem+Int+Ed+Engl 2014 ; 53 (38): 10242-6 Nephropedia Template TP {{tp|p=25081303|t=2014. Super-Resolution Imaging of the Golgi in Live Cells with a Bio-orthogonal Ceramide Probe** |pdf=|usr=}}{{25081303}} gab.com Text Super-Resolution Imaging of the Golgi in Live Cells with a Bio-orthogonal Ceramide Probe** JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=25081303 #FOAvip We report a lipid-based strategy to visualize Golgi structure and dynamics at super-resolution in live cells. The method is based on two novel reagents: a trans-cyclooctene-containing ceramide lipid (Cer-TCO) and a highly reactive, tetrazine-tagged near-IR dye (SiR-Tz). These reagents assemble via an extremely rapid ?tetrazine-click? reaction into Cer-SiR, a highly photostable ?vital dye? that enables prolonged live cell imaging of the Golgi apparatus by 3D confocal and STED microscopy. Cer-SiR is non-toxic at concentrations as high as 2 ?M and does not perturb the mobility of Golgi-resident enzymes or the traffic of cargo from the endoplasmic reticulum through the Golgi and to the plasma membrane. Twit Text FOAVip Super-Resolution Imaging of the Golgi in Live Cells with a Bio-orthogonal Ceramide Probe** ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=25081303 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25081303 #FOAvip English Wikipedia <ref>{{Cite pmid|25081303}}</ref> Super-Resolution Imaging of the Golgi in Live Cells with a Bio-orthogonal Ceramide Probe** #MMPMID25081303 Erdmann RS; Takakura H; Thompson AD; Rivera-Molina F; Allgeyer ES; Bewersdorf J; Toomre DK; Schepartz A Angew Chem Int Ed Engl 2014[Sep]; 53 (38): 10242-6 PMID25081303show ga We report a lipid-based strategy to visualize Golgi structure and dynamics at super-resolution in live cells. The method is based on two novel reagents: a trans-cyclooctene-containing ceramide lipid (Cer-TCO) and a highly reactive, tetrazine-tagged near-IR dye (SiR-Tz). These reagents assemble via an extremely rapid ?tetrazine-click? reaction into Cer-SiR, a highly photostable ?vital dye? that enables prolonged live cell imaging of the Golgi apparatus by 3D confocal and STED microscopy. Cer-SiR is non-toxic at concentrations as high as 2 ?M and does not perturb the mobility of Golgi-resident enzymes or the traffic of cargo from the endoplasmic reticulum through the Golgi and to the plasma membrane. äSuper-Resolution Imaging of the Golgi in Live Cells with a Bio-orthogo(epmc).pdf DeepDyve Pubget Overpricing