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2015 ; 5
(ä): 14706
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A new tool for long-term studies of POM-bacteria interactions: overcoming the
century-old Bottle Effect
#MMPMID26435525
Ionescu D
; Bizic-Ionescu M
; Khalili A
; Malekmohammadi R
; Morad MR
; de Beer D
; Grossart HP
Sci Rep
2015[Oct]; 5
(ä): 14706
PMID26435525
show ga
Downward fluxes of particulate organic matter (POM) are the major process for
sequestering atmospheric CO2 into aquatic sediments for thousands of years.
Budget calculations of the biological carbon pump are heavily based on the ratio
between carbon export (sedimentation) and remineralization (release to the
atmosphere). Current methodologies determine microbial dynamics on POM using
closed vessels, which are strongly biased towards heterotrophy due to rapidly
changing water chemistry (Bottle Effect). We developed a flow-through rolling
tank for long term studies that continuously maintains POM at near in-situ
conditions. There, bacterial communities resembled in-situ communities and
greatly differed from those in the closed systems. The active particle-associated
community in the flow-through system was stable for days, contrary to hours
previously reported for closed incubations. In contrast to enhanced respiration
rates, the decrease in photosynthetic rates on particles throughout the
incubation was much slower in our system than in traditional ones. These results
call for reevaluating experimentally-derived carbon fluxes estimated using
traditional methods.