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2014 ; 5
(1
): 167-98
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The First Order Transfer Function in the Analysis of Agrochemical Data in Honey
Bees (Apis Mellifera L ): Proboscis Extension Reflex (PER) Studies
#MMPMID26462584
De Stefano LA
; Stepanov II
; Abramson CI
Insects
2014[Jan]; 5
(1
): 167-98
PMID26462584
show ga
This paper describes a mathematical model of the learning process suitable for
studies of conditioning using the proboscis extension reflex (PER) in honey bees
when bees are exposed to agrochemicals. Although procedural variations exist in
the way laboratories use the PER paradigm, proboscis conditioning is widely used
to investigate the influence of pesticides and repellents on honey bee learning.
Despite the availability of several mathematical models of the learning process,
no attempts have been made to apply a mathematical model to the learning curve in
honey bees exposed to agrochemicals. Our model is based on the standard transfer
function in the form Y=B3 e(-B2 (X-1)) +B4(1-e(-B2 (X-1))) where X is the trial
number, Y is the proportion of correct responses, B2 is the learning rate, B3 is
readiness to learn, and B4 is ability to learn. We reanalyze previously published
data on the effect of several classes of agrochemicals including: (1) those that
are considered harmless to bees (e.g., pymetrozine, essential oils, dicofol); (2)
sublethal exposure to pesticides known to harm honey bees (e.g., coumaphos,
cyfluthrin, fluvalinate, permethrin); and (3) putative repellents of honey bees
(e.g., butyric acid, citronella). The model revealed additional effects not
detected with standard statistical tests of significance.