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10.1063/1.4931638 http://scihub22266oqcxt.onion/10.1063/1.4931638 C4592426!4592426!26487897     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomicrofluidics 2015 ; 9 (5): ä Nephropedia Template TP {{tp|p=26487897|t=2015. Flip channel: A microfluidic device for uniform-sized embryoid body formation and differentiation |pdf=|usr=}}{{26487897}} gab.com Text Flip channel: A microfluidic device for uniform-sized embryoid body formation and differentiation JO: Biomicrofluidics http://www.kidney.de/pget.php?&tw=1&pmid=26487897 #FOAvip This paper reports a two-layered polydimethylsiloxane microfluidic device?Flip channel, capable of forming uniform-sized embryoid bodies (EBs) and performing stem cell differentiation within the same device after flipping the microfluidic channel. The size of EBs can be well controlled by designing the device geometries, and EBs with multiple sizes can be formed within a single device to study EB size-dependent stem cell differentiation. During operation of the device, cells are positioned in the designed positions. As a result, observation and monitoring specific population of cells can be achieved for further analysis. In addition, after flipping the microfluidic channel, stem cell differentiation from the EBs can be performed on an unconfined flat surface that is desired for various differentiation processes. In the experiments, murine embryonic stem cells (ES-D3) are cultured and formed EBs inside the developed device. The size of EBs is well controlled inside the device, and the neural differentiation is performed on the formed EBs after flipping the channel. The EB size-dependent stem cell differentiation is studied using the device to demonstrate its functions. The device provides a useful tool to study stem cell differentiation without complicated device fabrication and tedious cell handling under better-controlled microenvironments. Twit Text FOAVip Flip channel: A microfluidic device for uniform-sized embryoid body formation and differentiation ????Biomicrofluidics http://www.kidney.de/pget.php?&tw=1&pmid=26487897 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26487897 #FOAvip English Wikipedia <ref>{{Cite pmid|26487897}}</ref> Flip channel: A microfluidic device for uniform-sized embryoid body formation and differentiation #MMPMID26487897 Chen YH; Peng CC; Tung YC Biomicrofluidics 2015[Sep]; 9 (5): ä PMID26487897show ga This paper reports a two-layered polydimethylsiloxane microfluidic device?Flip channel, capable of forming uniform-sized embryoid bodies (EBs) and performing stem cell differentiation within the same device after flipping the microfluidic channel. The size of EBs can be well controlled by designing the device geometries, and EBs with multiple sizes can be formed within a single device to study EB size-dependent stem cell differentiation. During operation of the device, cells are positioned in the designed positions. As a result, observation and monitoring specific population of cells can be achieved for further analysis. In addition, after flipping the microfluidic channel, stem cell differentiation from the EBs can be performed on an unconfined flat surface that is desired for various differentiation processes. In the experiments, murine embryonic stem cells (ES-D3) are cultured and formed EBs inside the developed device. The size of EBs is well controlled inside the device, and the neural differentiation is performed on the formed EBs after flipping the channel. The EB size-dependent stem cell differentiation is studied using the device to demonstrate its functions. The device provides a useful tool to study stem cell differentiation without complicated device fabrication and tedious cell handling under better-controlled microenvironments. äFlip channel: A microfluidic device for uniform-sized embryoid body fo(epmc).pdf DeepDyve Pubget Overpricing