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10.1152/ajpheart.00095.2015 http://scihub22266oqcxt.onion/10.1152/ajpheart.00095.2015 C4591410!4591410 !26116710     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26116710 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Am+J+Physiol+Heart+Circ+Physiol 2015 ; 309 (5 ): H880-7 Nephropedia Template TP {{tp|p=26116710 |t=2015. Activation of the (pro)renin receptor in the paraventricular nucleus increases sympathetic outflow in anesthetized rats |pdf=|usr=}}{{26116710 }} gab.com Text Activation of the (pro)renin receptor in the paraventricular nucleus increases sympathetic outflow in anesthetized rats JO: Am J Physiol Heart Circ Physiol http://www.kidney.de/pget.php?&tw=1&pmid=26116710 #FOAvip Previous studies have indicated that hyperactivity of brain prorenin receptors (PRR) is implicated in neurogenic hypertension. However, the role of brain PRR in regulating arterial blood pressure (ABP) is not well understood. Here, we test the hypothesis that PRR activation in the hypothalamic paraventricular nucleus (PVN) contributes to increased sympathetic nerve activity (SNA). In anaesthetized adult Sprague-Dawley (SD) rats, bilateral PVN microinjection of human prorenin (2 pmol/side) significantly increased splanchnic SNA (SSNA; 71 ± 15%, n = 7). Preinjection of either prorenin handle region peptide, the PRR binding blocker (PRRB), or tiron (2 nmol/side), the scavenger of reactive oxygen species (ROS), significantly attenuated the increase in SSNA (PRRB: 32 ± 5% vs. control, n = 6; tiron: 8 ± 10% vs. control, n = 5; P < 0.05) evoked by prorenin injection. We further investigated the effects of PRR activation on ROS production as well as downstream gene expression using cultured hypothalamus neurons from newborn SD rats. Incubation of brain neurons with human prorenin (100 nM) dramatically enhanced ROS production and induced a time-dependent increase in mRNA levels of inducible nitric oxide synthase (iNOS), NAPDH oxidase 2 subunit cybb, and FOS-like antigen 1 (fosl1), a marker for neuronal activation and a component of transcription factor activator protein-1 (AP-1). The maximum mRNA increase in these genes occurred 6 h following incubation (iNOS: 201-fold; cybb: 2 -fold; Ffosl1: 11-fold). The increases in iNOS and cybb mRNA were not attenuated by the AT1 receptor antagonist losartan but abolished by the AP-1 blocker curcumin. Our results suggest that PVN PRR activation induces sympathoexcitation possibly through stimulation of an ANG II-independent, ROS-AP-1-iNOS signaling pathway. Twit Text FOAVip Activation of the (pro)renin receptor in the paraventricular nucleus increases sympathetic outflow in anesthetized rats ????Am J Physiol Heart Circ Physiol http://www.kidney.de/pget.php?&tw=1&pmid=26116710 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26116710 #FOAvip English Wikipedia <ref>{{Cite pmid|26116710 }}</ref> Activation of the (pro)renin receptor in the paraventricular nucleus increases sympathetic outflow in anesthetized rats #MMPMID26116710 Huber MJ ; Basu R ; Cecchettini C ; Cuadra AE ; Chen QH ; Shan Z Am J Physiol Heart Circ Physiol 2015[Sep]; 309 (5 ): H880-7 PMID26116710 show ga Previous studies have indicated that hyperactivity of brain prorenin receptors (PRR) is implicated in neurogenic hypertension. However, the role of brain PRR in regulating arterial blood pressure (ABP) is not well understood. Here, we test the hypothesis that PRR activation in the hypothalamic paraventricular nucleus (PVN) contributes to increased sympathetic nerve activity (SNA). In anaesthetized adult Sprague-Dawley (SD) rats, bilateral PVN microinjection of human prorenin (2 pmol/side) significantly increased splanchnic SNA (SSNA; 71 ± 15%, n = 7). Preinjection of either prorenin handle region peptide, the PRR binding blocker (PRRB), or tiron (2 nmol/side), the scavenger of reactive oxygen species (ROS), significantly attenuated the increase in SSNA (PRRB: 32 ± 5% vs. control, n = 6; tiron: 8 ± 10% vs. control, n = 5; P < 0.05) evoked by prorenin injection. We further investigated the effects of PRR activation on ROS production as well as downstream gene expression using cultured hypothalamus neurons from newborn SD rats. Incubation of brain neurons with human prorenin (100 nM) dramatically enhanced ROS production and induced a time-dependent increase in mRNA levels of inducible nitric oxide synthase (iNOS), NAPDH oxidase 2 subunit cybb, and FOS-like antigen 1 (fosl1), a marker for neuronal activation and a component of transcription factor activator protein-1 (AP-1). The maximum mRNA increase in these genes occurred 6 h following incubation (iNOS: 201-fold; cybb: 2 -fold; Ffosl1: 11-fold). The increases in iNOS and cybb mRNA were not attenuated by the AT1 receptor antagonist losartan but abolished by the AP-1 blocker curcumin. Our results suggest that PVN PRR activation induces sympathoexcitation possibly through stimulation of an ANG II-independent, ROS-AP-1-iNOS signaling pathway. |Action Potentials [MESH] |Anesthesia [MESH] |Animals [MESH] |Blood Pressure [MESH] |Cells, Cultured [MESH] |Humans [MESH] |Hypothalamus/cytology/metabolism [MESH] |Male [MESH] |Membrane Glycoproteins/genetics/metabolism [MESH] |NADPH Oxidase 2 [MESH] |NADPH Oxidases/genetics/metabolism [MESH] |Neurons/drug effects/metabolism/physiology [MESH] |Nitric Oxide Synthase Type II/genetics/metabolism [MESH] |Paraventricular Hypothalamic Nucleus/*metabolism/physiology [MESH] |Prorenin Receptor [MESH] |Proto-Oncogene Proteins c-fos/genetics/metabolism [MESH] |RNA, Messenger/genetics/metabolism [MESH] |Rats [MESH] |Rats, Sprague-Dawley [MESH] |Reactive Oxygen Species/metabolism [MESH] |Receptors, Cell Surface/*metabolism [MESH] |Renin/pharmacology [MESH] |Sympathetic Nervous System/metabolism/*physiology [MESH]Activation of the (pro)renin receptor in the paraventricular nucleus i(epmc).pdf DeepDyve Pubget Overpricing